Jonathan Mill, Philip Asherson, and Ian Craig contributed equally to this work.
Expression of the dopamine transporter gene is regulated by the 3′ UTR VNTR: Evidence from brain and lymphocytes using quantitative RT-PCR†
Article first published online: 7 OCT 2002
Copyright © 2002 Wiley-Liss, Inc.
American Journal of Medical Genetics
Volume 114, Issue 8, pages 975–979, 8 December 2002
How to Cite
Mill, J., Asherson, P., Browes, C., D'Souza, U. and Craig, I. (2002), Expression of the dopamine transporter gene is regulated by the 3′ UTR VNTR: Evidence from brain and lymphocytes using quantitative RT-PCR. Am. J. Med. Genet., 114: 975–979. doi: 10.1002/ajmg.b.10948
- Issue published online: 26 NOV 2002
- Article first published online: 7 OCT 2002
- Manuscript Accepted: 7 AUG 2002
- Manuscript Received: 6 JUN 2002
- dopamine transporter gene (DAT1);
- quantitative RT-PCR;
- attention deficit hyperactivity disorder
Genetic association studies provide considerable evidence that the 10-repeat allele of a variable number tandem repeat (VNTR) in the 3′-untranslated region (3′-UTR) of the dopamine transporter gene (DAT1) is associated with a range of psychiatric phenotypes, most notably, attention deficit hyperactivity disorder. The mechanism for this association is not yet understood, although several lines of evidence implicate variation in gene expression. In this study, we measured DAT1 messenger RNA levels in cerebellum, temporal lobe, and lymphocytes using quantitative real-time reverse-transcription polymerase chain reaction. Relative to a set of four control housekeeping genes (β-actin, GAPD, ribosomal 18S, and β2-microglobulin) we observed that increased levels of DAT1 expression were associated with the number of 10-repeat alleles. These data provide direct evidence that the VNTR, or another polymorphism in linkage disequilibrium with the VNTR, is involved in regulating expression of this gene. © 2002 Wiley-Liss, Inc.