Research Article

Transcriptional profiling of subjects from the Iowa adoption studies†

  1. Robert A. Philibert1,2,*,
  2. Gi-Yung Ryu3,
  3. Jae-Geun Yoon3,
  4. Harinder Sandhu1,
  5. Nancy Hollenbeck1,
  6. Tracy Gunter1,
  7. Amanda Barkhurst1,
  8. William Adams1,
  9. Anup Madan3

Article first published online: 6 MAR 2007

DOI: 10.1002/ajmg.b.30512

Issue

American Journal of Medical Genetics Part B: Neuropsychiatric Genetics

American Journal of Medical Genetics Part B: Neuropsychiatric Genetics

Volume 144B, Issue 5, pages 683–690, 5 July 2007

Additional Information

How to Cite

Philibert, R. A., Ryu, G.-Y., Yoon, J.-G., Sandhu, H., Hollenbeck, N., Gunter, T., Barkhurst, A., Adams, W. and Madan, A. (2007), Transcriptional profiling of subjects from the Iowa adoption studies. American Journal of Medical Genetics Part B: Neuropsychiatric Genetics, 144B: 683–690. doi: 10.1002/ajmg.b.30512

Author Information

  1. 1

    Department of Psychiatry, The University of Iowa, Iowa City, Iowa

  2. 2

    Neuroscience Program, The University of Iowa, Iowa City, Iowa

  3. 3

    Department of Neurosurgery, The University of Iowa, Iowa City, Iowa

*The University of Iowa, Rm 2-126 MEB, Iowa City, IA.

  1. †

    Please cite this article as follows: Philibert RA, Ryu G-Y, Yoon J-G, Sandhu H, Hollenbeck N, Gunter T, Barkhurst A, Adams W, Madan A. 2007. Transcriptional Profiling of Subjects From the Iowa Adoption Studies. Am J Med Genet Part B 144B:683–690.

Publication History

  1. Issue published online: 21 JUN 2007
  2. Article first published online: 6 MAR 2007
  3. Manuscript Accepted: 18 JAN 2007
  4. Manuscript Received: 11 JUL 2006

Funded by

  • NIH. Grant Numbers: K08MH064714, R01DA015789, R01AI053264

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (186K)

Keywords:

  • substance use;
  • major depression;
  • SLC6A4;
  • AUTS2;
  • CAPN2;
  • ELN;
  • lymphoblast

Abstract

Transcriptional profiling has been used to identify gene expression patterns indicative of general medical illnesses such as atherosclerosis. However, whether these methods can identify common psychiatric disorders has not been established. To answer this question with respect to nicotine use, we used genome-wide expression profiling lymphoblast cell lines from six actively smoking Iowa Adoption Studies (IAS) subjects and nine “clean” control subjects, followed by real-time PCR (RT-PCR) of gene expression patterns in lymphoblast derived RNA from 94 subjects in the IAS. As compared to those from controls without a history of smoking (n = 9), the expression levels of 579 of 29,098 genes were significantly up-regulated and expression levels of 584 of 29,098 genes were significantly down-regulated in lymphoblast lines from currently smoking subjects (n = 6). RT-PCR confirmation of four select RNA levels confirmed the validity of the overall profile and revealed highly significant relationships between the expression of some of these transcripts and (1) major depression, (2) antisocial personality, (3) nicotine dependence, and (4) cannabis dependence. We conclude that the use of expression profiling may contribute significant insights into the biology of complex behavioral disorders. © 2007 Wiley-Liss, Inc.

View Full Article (HTML) Get PDF (186K)