How to Cite this Article: Zubenko GS, Hughes HB. 2012. No Evidence of Non-Homologous Insertions in Mouse Model of MDD Created by Replacement of Homologous Mouse DNA Sequence With Pathogenic 6-Base Human CREB1 Promoter Sequence. Am J Med Genet Part B 159B:1–4.
No evidence of non-homologous insertions in mouse model of MDD created by replacement of homologous mouse DNA sequence with pathogenic 6-base human CREB1 promoter sequence†
Article first published online: 16 DEC 2011
Copyright © 2011 Wiley Periodicals, Inc.
American Journal of Medical Genetics Part B: Neuropsychiatric Genetics
Volume 159B, Issue 1, pages 1–4, January 2012
How to Cite
Zubenko, G. S. and Hughes, H. B. (2012), No evidence of non-homologous insertions in mouse model of MDD created by replacement of homologous mouse DNA sequence with pathogenic 6-base human CREB1 promoter sequence. Am. J. Med. Genet., 159B: 1–4. doi: 10.1002/ajmg.b.32006
- Issue published online: 16 DEC 2011
- Article first published online: 16 DEC 2011
- Manuscript Accepted: 16 NOV 2011
- Manuscript Received: 7 OCT 2011
- Provost's Fund for Research Development, University of Pittsburgh
- Shane Richard Brown Fund, University of Pittsburgh
- Office of the Senior Vice Chancellor for the Health Sciences, University of Pittsburgh
- National Center for Research Resources (NCRR). Grant Number: UL1 RR024153
- major depressive disorder;
- animal model
We have recently reported the creation and initial characterization of the first etiology-based recombinant mouse model of major depressive disorder (MDD). This was achieved by replacing the corresponding mouse DNA sequence with a 6-base DNA sequence from the human CREB1 promoter that is associated with the development of MDD in families identified by probands with recurrent, early-onset MDD. The current study explored whether the desired homologous recombination event at the mouse Creb1 gene that resulted in the creation of the mouse model was also accompanied by insertions of the targeting vector at unintended non-homologous locations in the mouse genome. No evidence of insertions of targeting vector sequence was observed at regions other than the mouse Creb1 gene. © 2011 Wiley Periodicals, Inc.