Petra Platte, Ph.D., is a research scientist at Biological and Clinical Psychology, University of Wuerzburg, Wuerzburg, Germany. She joined the University of Pennsylvania, PA from 1995 to 1998. Together with Drs. Papanicolaou, Stunkard, and Wilson she continues studies in the Old Order Amish.
A study of linkage and association of body mass index in the old order Amish†
Article first published online: 26 JUN 2003
Published 2003 Wiley-Liss, Inc.
American Journal of Medical Genetics Part C: Seminars in Medical Genetics
Special Issue: Medical Genetics Studies in the Amish
Volume 121C, Issue 1, pages 71–80, 15 August 2003
How to Cite
Platte, P., Papanicolaou, G.J., Johnston, J., Klein, C.M., Doheny, K.F., Pugh, E.W., Roy-Gagnon, M.-H., Stunkard, A.J., Francomano, C.A. and Wilson, A.F. (2003), A study of linkage and association of body mass index in the old order Amish. Am. J. Med. Genet., 121C: 71–80. doi: 10.1002/ajmg.c.20005
This article was prepared by a group consisting of both United States Government employees and non-United States Government employees, and as such is subject to 117 U.S.C. Sec. 105.
- Issue published online: 9 JUL 2003
- Article first published online: 26 JUN 2003
- Deutsche Forschungsgesellschaft. Grant Number: PL 216/2-1
- body mass index;
Obesity is thought to have a genetic component with the estimates of heritability ranging from 0.25–0.40. As part of an ongoing study of obesity in the Old Order Amish, seven two- and three-generation families (157 individuals) were assessed for 21 traits related to obesity, including body mass index (BMI) and BMI-percentile (a standardized distribution of BMI adjusted for age and sex). Genotyping was performed using a panel of 384 short-tandem repeat markers. In this sample, the estimates of heritability ranged from 0.16–0.31 for BMI and from 0.40–0.52 for BMI-percentile. Model-independent linkage analysis identified candidate regions on chromosomes 1, 5, 7, 8, and 11. Given that several markers on 7q were significant for both BMI and BMI-percentile (P ≤ 0.001) and that the structural locus for leptin was located on 7q, this region was considered to be the primary candidate region. Subsequent typing of additional flanking markers on 7q corroborated the original findings. Tests of intrafamilial association for alleles at markers in this candidate region were significant at similar levels. Although there is some evidence for linkage and association in the region containing leptin, there appears to be stronger evidence for linkage (P ≤ 0.001) and association (P ≤ 0.00001) with BMI in a region 10–15 cM further downstream of leptin, flanked by markers D7S1804 and D7S3070 with peak values from D7S495–D7S1798. Evidence from linkage and association studies suggests that this region (D7S1804–D7S3070) may be responsible, at least in part, for variation in BMI and BMI-percentile in the Old Order Amish. Published 2003 Wiley-Liss, Inc.