Prometaphase chromosomes of the howler monkey (Aloutta caraya): G, C, NOR, and restriction enzyme (RES) banding

Authors

  • M. Mudry,

    Corresponding author
    1. GIBE (Grupo de Investigoción en Biologia Evolutiva), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturcles (FCE y N), Universidad de Buenos Aires (UBA), Buenos Aires
    • GIBE, Departamento de Ciencias Biol6gicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellòn 2.(4°- piso), Nuñez, (1428) Capital Federal, Buenos Aires, Argentina
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  • M. Ponsà,

    1. Departmento de Biologia Cellular i Fisiologia and Institut de Biologia Fonamental “Vicent Villar Palasí,” Uniuersitat Autònoma de Barcelona, Spain
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  • A. Borrell,

    1. Departmento de Biologia Cellular i Fisiologia. Universitat Autònoma de Barcelona, Barcelona, Spain
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  • J. Egozcue,

    1. Departmento de Biologia Cellular i Fisiologia and Institut de Biologia Fonamental “Vicent Villar Palasí,” Uniuersitat Autònoma de Barcelona, Spain
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  • M. Garcia

    1. Departmento de Biologia Cellular i Fisiologia and Institut de Biologia Fonamental “Vicent Villar Palasí,” Uniuersitat Autònoma de Barcelona, Spain
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Abstract

Prometaphase lymphocyte chromosomes from eight adult argentinian Alouatta caraya females were characterized using sequential G-C banding techniques, Ag-NOR bands and bands obtained with the restriction enzymes Hae III, Eco RI, Alu I and Sau 3A.

The cytogenetic analysis showed 2n = 52, with four, five, or six NOR chromosomes. Digestion with Hae III and Eco RI produced G-like-bands. Centromere regions and two interstitial C-bands (in chromosomes number 16 and 21) showed intraindividual or interindividual heterochromatic polymorphisms. Alu I digestion produced C-like bands with gaps in the centromere regions, and Sau 3A produced C-like bands. The karyotypes and banding patterns of A. caraya, A. palliata, A. belzebul, and A. seniculus are compared, based on whole chromosome and whole arm homeologies. © 1994 Wiley-Liss, Inc.

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