Cross-species amplification of human microsatellite markers using noninvasive samples from white-handed gibbons (Hylobates lar)
Article first published online: 3 SEP 2004
© 2004 Wiley-Liss, Inc.
American Journal of Primatology
Volume 64, Issue 1, pages 19–27, September 2004
How to Cite
Chambers, K. E., Reichard, U. H., Möller, A., Nowak, K. and Vigilant, L. (2004), Cross-species amplification of human microsatellite markers using noninvasive samples from white-handed gibbons (Hylobates lar). Am. J. Primatol., 64: 19–27. doi: 10.1002/ajp.20058
- Issue published online: 3 SEP 2004
- Article first published online: 3 SEP 2004
- Manuscript Accepted: 14 JUN 2004
- Manuscript Revised: 28 MAY 2004
- Manuscript Received: 21 JAN 2004
- Max Planck Society
- cross-species amplification;
Analysis of the population genetic structure and reproductive strategies of various primate species has been facilitated by cross-species amplification (i.e., the use of microsatellite markers developed in one species for analysis of another). In this study we screened 47 human-derived markers to assess their utility in the white-handed gibbon (Hylobates lar). Only eight produced accurate, reliable results, and exhibited levels of polymorphism that were adequate for individual identification. This low success rate was surprising given that human microsatellite markers typically work well in species (such as macaques) that are evolutionarily more distant from humans than are gibbons. In addition, we experienced limited success in using a set of microsatellite markers that have been reported to be useful in the closely-related H. muelleri, and applying our set of microsatellite markers to samples obtained from one H. pileatus individual. Our results emphasize the importance of extensively screening potential markers in representatives of the population of interest. Am. J. Primatol. 64:19–27, 2004. © 2004 Wiley-Liss, Inc.