Increased positive selection pressure within the complementarity determining regions of the T-cell receptor β gene in New World monkeys

Authors

  • Takaji Matsutani,

    1. Laboratory of Immune Regulation, Wakayama Medical University, Osaka, Japan
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    • b

      Takaji Matsutani and Yoshiki Fujii contributed equally to this work.

  • Yoshiki Fujii,

    1. Department of Rheumatology and Clinical Immunology, Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Kanagawa, Japan
    2. Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan
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    • b

      Takaji Matsutani and Yoshiki Fujii contributed equally to this work.

  • Kazutaka Kitaura,

    1. Department of Rheumatology and Clinical Immunology, Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Kanagawa, Japan
    2. Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan
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  • Satsuki Suzuki,

    1. Section of Biological Science, Research Center for Odontology, Nippon Dental University School of Life Dentistry, Tokyo, Japan
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  • Yuji Tsuruta,

    1. Discovery Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan
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  • Tomohiko Takasaki,

    1. Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan
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  • Kouetsu Ogasawara,

    1. Department of Immunobiology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
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  • Norihiro Nishimoto,

    1. Laboratory of Immune Regulation, Wakayama Medical University, Osaka, Japan
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  • Ichiro Kurane,

    1. Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan
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  • Ryuji Suzuki

    Corresponding author
    1. Department of Rheumatology and Clinical Immunology, Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Kanagawa, Japan
    • Department of Rheumatology and Clinical Immunology, Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, National Hospital Organization, 18-1 Sakuradai, Minami-ku, Sagamihara, Kanagawa 228-0815, Japan
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Abstract

Because of the long-term co-evolution of TCR and MHC molecules, numerous nucleotide substitutions have accumulated within the domains of TCRβ genes. We previously found that nonsynonymous nucleotide substitutions occurred more frequently in complementarity determining region (CDR)β than in CDRα, even though only a limited number of common marmoset (Callithrix jacchus) and human T-cell receptor β variable (TRBV) sequences were compared. This interesting finding raised the question of whether the increased selective pressure within CDRβ was species-specific. In this study, we identified 21 TRBV region sequences from the common marmoset and performed comparative sequence analyses of the T-cell receptor α variable (TRAV) and TRBV regions from human, chimpanzee, rhesus monkey, cotton-top tamarin, Ma's night monkey, and common marmoset. The ratios of the number of nonsynonymous nucleotide substitutions per site (dN) to the dS values (dN/dS) were less than 1 within the framework regions (FRs) of TRAV and TRBV region sequences, suggesting that purifying selection is largely dominant within the FRs. In contrast, the dN values were statistically significantly greater for CDRβ than for CDRα only in New World monkeys. Also, increased dN/dS ratios (dN/dS>1) were observed within CDRβ between humans and New World monkeys and, interestingly, between New World monkeys, which share a relatively recent common ancestor. Moreover, phylogenetic analysis by maximum likelihood analysis provided firm evidence to support that positive selection occurred within CDRβ along New World monkey lineages. These results suggest that increased positive selection pressure within CDRβ is common in New World monkeys rather than being species-specific. This study provides an intriguing insight into the co-evolution of TCR and MHC molecules within primates. Am. J. Primatol. 73:1082–1092, 2011. © 2011 Wiley-Liss, Inc.

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