Contract grant sponsor: NIH-NCRR; Contract grant numbers: P51 RR000166; RR 02S014; Contract grant sponsor: NIH-NIAID; Contract grant numbers: R01 AI078229; R03 AI064865; Contract grant sponsor: DARPA; Contract grant number: N66001-02-C-8072; Contract grant sponsor: Unconventional Concepts Inc; contract grant sponsor: Chicago Zoological Society; contract grant sponsor: University of New Mexico Research Allocations Committee.
From the Mouths of Monkeys: Detection of Mycobacterium tuberculosis Complex DNA From Buccal Swabs of Synanthropic Macaques
Article first published online: 29 MAY 2012
© 2012 Wiley Periodicals, Inc.
American Journal of Primatology
Volume 74, Issue 7, pages 676–686, July 2012
How to Cite
WILBUR, A. K., ENGEL, G. A., ROMPIS, A., PUTRA, I.G.A. A., LEE, B. P. Y.-H., AGGIMARANGSEE, N., CHALISE, M., SHAW, E., OH, G., SCHILLACI, M. A. and JONES-ENGEL, L. (2012), From the Mouths of Monkeys: Detection of Mycobacterium tuberculosis Complex DNA From Buccal Swabs of Synanthropic Macaques. Am. J. Primatol., 74: 676–686. doi: 10.1002/ajp.22022
- Issue published online: 29 MAY 2012
- Article first published online: 29 MAY 2012
- Manuscript Accepted: 1 FEB 2012
- Manuscript Revised: 26 JAN 2012
- Manuscript Received: 14 NOV 2011
- NIH-NCRR. Grant Number: P51 RR000166; RR 02S014
- NIH-NIAID. Grant Number: R01 AI078229; R03 AI064865
- DARPA. Grant Number: N66001-02-C-8072
- Unconventional Concepts Inc
- Chicago Zoological Society
- University of New Mexico Research Allocations Committee
- nonhuman primate;
- Mycobacterium tuberculosis complex, MTBC;
- Southeast Asia;
Although the Mycobacterium tuberculosis complex (MTBC) infects a third of all humans, little is known regarding the prevalence of mycobacterial infection in nonhuman primates (NHP). For more than a century, tuberculosis has been regarded as a serious infectious threat to NHP species. Advances in the detection of MTBC open new possibilities for investigating the effects of this poorly understood pathogen in diverse populations of NHP. Here, we report results of a cross-sectional study using well-described molecular methods to detect a nucleic acid sequence (IS6110) unique to the MTBC. Sample collection was focused on the oral cavity, the presumed route of transmission of MTBC. Buccal swabs were collected from 263 macaques representing 11 species in four Asian countries and Gibraltar. Contexts of contact with humans included free ranging, pets, performing monkeys, zoos, and monkey temples. Following DNA isolation from buccal swabs, the polymerase chain reaction (PCR) amplified IS6110 from 84 (31.9%) of the macaques. In general, prevalence of MTBC DNA was higher among NHP in countries where the World Health Organization reports higher prevalence of humans infected with MTBC. This is the first demonstration of MTBC DNA in the mouths of macaques. Further research is needed to establish the significance of this finding at both the individual and population levels. PCR of buccal samples holds promise as a method to elucidate the mycobacterial landscape among NHP, particularly macaques that thrive in areas of high human MTBC prevalence. Am. J. Primatol. 74:676–686, 2012. © 2012 Wiley Periodicals, Inc.