The first two authors contributed equally to the manuscript.Contract grant sponsor: National Institutes of Health; Contract grant number: K99HD057992-02; Contract grant sponsor: Davee Foundation; Contract grant sponsor: Guthman Foundation; Contract grant sponsor: Leakey Foundation; Contract grant sponsor: Wenner-Gren Foundation; Contract grant sponsor: NSFGRF.
Validation of a Field Technique and Characterization of Fecal Glucocorticoid Metabolite Analysis in Wild Chimpanzees (Pan troglodytes)
Version of Record online: 11 SEP 2012
© 2012 Wiley Periodicals, Inc.
American Journal of Primatology
Volume 75, Issue 1, pages 57–64, January 2013
How to Cite
MURRAY, C. M., HEINTZ, M. R., LONSDORF, E. V., PARR, L. A. and SANTYMIRE, R. M. (2013), Validation of a Field Technique and Characterization of Fecal Glucocorticoid Metabolite Analysis in Wild Chimpanzees (Pan troglodytes). Am. J. Primatol., 75: 57–64. doi: 10.1002/ajp.22078
- Issue online: 18 DEC 2012
- Version of Record online: 11 SEP 2012
- Manuscript Accepted: 9 AUG 2012
- Manuscript Revised: 19 JUL 2012
- Manuscript Received: 27 MAR 2012
- National Institutes of Health. Grant Number: K99HD057992-02
- Davee Foundation
- Guthman Foundation
- Leakey Foundation
- Wenner-Gren Foundation
- fecal glucocorticoid metabolites;
- field methods;
- circadian rhythm
Monitoring adrenocortical activity in wild primate populations is critical, given the well-documented relationship between stress, health, and reproduction. Although many primate studies have quantified fecal glucocorticoid metabolite (FGM) concentrations, it is imperative that researchers validate their method for each species. Here, we describe and validate a technique for field extraction and storage of FGMs in wild chimpanzees (Pan troglodytes). Our method circumvents many of the logistical challenges associated with field studies while yielding similar results to a commonly used laboratory method. We further validate that our method accurately reflects stress physiology using an adrenocorticotropic hormone challenge in a captive chimpanzee and an FGM peak at parturition in a wild subject. Finally, we quantify circadian patterns for FGMs for the first time in this species. Understanding these patterns may allow researchers to directly link specific events with the stress response. Am. J. Primatol. 75:57-64, 2013. © 2012 Wiley Periodicals, Inc.