Technical note: Improved ancient DNA purification for PCR using ion-exchange columns

Authors

  • Kijeong Kim,

    1. Institute for Medical Sciences, Chung-Ang University, Seoul 156-756, South Korea
    2. Department of Microbiology, College of Medicine, Chung-Ang University, Seoul 156-756, South Korea
    3. Department of Cultural Properties, Chung-Ang University, Seoul 156-756, South Korea
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  • Kyung-Yong Kim,

    1. Institute for Medical Sciences, Chung-Ang University, Seoul 156-756, South Korea
    2. Department of Cultural Properties, Chung-Ang University, Seoul 156-756, South Korea
    3. Department of Anatomy, College of Medicine, Chung-Ang University, Seoul 156-756, South Korea
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  • Eunhee Jeon,

    1. Department of Microbiology, College of Medicine, Chung-Ang University, Seoul 156-756, South Korea
    2. Department of Cultural Properties, Chung-Ang University, Seoul 156-756, South Korea
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  • Ariunaa Togloom,

    1. Department of Microbiology, College of Medicine, Chung-Ang University, Seoul 156-756, South Korea
    2. Department of Cultural Properties, Chung-Ang University, Seoul 156-756, South Korea
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  • Youn-Ock Cho,

    1. Department of Cultural Properties, Chung-Ang University, Seoul 156-756, South Korea
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  • Min-Soo Lee,

    1. Department of Cultural Properties, Chung-Ang University, Seoul 156-756, South Korea
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  • Gavaachimed Lkhagvasuren,

    1. Department of Cultural Properties, Chung-Ang University, Seoul 156-756, South Korea
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  • Jee-Hye Choi,

    1. Department of Life Science, College of Natural Science, Chung-Ang University, Seoul 156-756, South Korea
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  • Dashtseveg Tumen,

    1. Department of Anthropology and Archeology, School of Social Sciences, National University of Mongolia, Ulaanbaatar, Mongolia
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  • Ae Ja Park,

    1. Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul 156-756, South Korea
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  • Keun-Cheol Kim,

    1. Department of Biology, Institute for Life Science, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, South Korea
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  • Ki-Won Park,

    1. DNA Analysis Section, National Institute of Scientific Investigation, Seoul 158-707, South Korea
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  • Jae-Hyun Kim,

    1. Department of Archaelogy and Art History, College of Humanities, Donga University, Pusan 604-714, South Korea
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  • Maengseok Noh,

    1. Division of Mathematical Science, College of Natural Sciences, Pukyong National University, Pusan 608-737, South Korea
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  • Kwon-Jong Yoo,

    1. Department of Cultural Properties, Chung-Ang University, Seoul 156-756, South Korea
    2. Department of Philosophy, College of Liberal Arts, Chung-Ang University, Seoul 156-756, South Korea
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  • Kwang-Ho Lee

    Corresponding author
    1. Department of Cultural Properties, Chung-Ang University, Seoul 156-756, South Korea
    2. Department of Life science, Chung-Ang University, Seoul 156-756, South Korea
    • Department of Life Science and Department of Cultural Properties, Chung-Ang University, 221, Heukseok-dong, Dongjak-gu, Seoul 156-756, South Korea
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Abstract

A novel method of ancient DNA (aDNA) purification was developed using ion-exchange columns to improve PCR-amplifiable DNA extraction from ancient bone samples. Thirteen PCR-resistant ancient bone samples aged 500–3,300 years were tested to extract aDNA using a recently reported, silica-based aDNA extraction method and an ion-exchange column method for the further purification. The PCR success rates of the aDNA extracts were evaluated for the amplification ability of the fragments of mitochondrial DNA, a high-copy DNA, and amelogenin, a low-copy DNA. The results demonstrate that the further purification of silica-based aDNA extracts using ion-exchange columns considerably improved PCR amplification. We suggest that the ion-exchange column-based method will be useful for the improvement of PCR-amplifiable aDNA extraction, particularly from the poorly preserved, PCR-resistant, ancient samples. Am J Phys Anthropol, 2008. © 2008 Wiley-Liss, Inc.

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