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Technical Note

Technical note: Improved ancient DNA purification for PCR using ion-exchange columns

  1. Kijeong Kim1,2,3,
  2. Kyung-Yong Kim1,3,4,
  3. Eunhee Jeon2,3,
  4. Ariunaa Togloom2,3,
  5. Youn-Ock Cho3,
  6. Min-Soo Lee3,
  7. Gavaachimed Lkhagvasuren3,
  8. Jee-Hye Choi5,
  9. Dashtseveg Tumen6,
  10. Ae Ja Park7,
  11. Keun-Cheol Kim8,
  12. Ki-Won Park9,
  13. Jae-Hyun Kim10,
  14. Maengseok Noh11,
  15. Kwon-Jong Yoo3,12,
  16. Kwang-Ho Lee3,13,*

Article first published online: 6 FEB 2008

DOI: 10.1002/ajpa.20782

Issue

American Journal of Physical Anthropology

American Journal of Physical Anthropology

Volume 136, Issue 1, pages 114–121, May 2008

Additional Information

How to Cite

Kim, K., Kim, K.-Y., Jeon, E., Togloom, A., Cho, Y.-O., Lee, M.-S., Lkhagvasuren, G., Choi, J.-H., Tumen, D., Ja Park, A., Kim, K.-C., Park, K.-W., Kim, J.-H., Noh, M., Yoo, K.-J. and Lee, K.-H. (2008), Technical note: Improved ancient DNA purification for PCR using ion-exchange columns. Am. J. Phys. Anthropol., 136: 114–121. doi: 10.1002/ajpa.20782

Author Information

  1. 1

    Institute for Medical Sciences, Chung-Ang University, Seoul 156-756, South Korea

  2. 2

    Department of Microbiology, College of Medicine, Chung-Ang University, Seoul 156-756, South Korea

  3. 3

    Department of Cultural Properties, Chung-Ang University, Seoul 156-756, South Korea

  4. 4

    Department of Anatomy, College of Medicine, Chung-Ang University, Seoul 156-756, South Korea

  5. 5

    Department of Life Science, College of Natural Science, Chung-Ang University, Seoul 156-756, South Korea

  6. 6

    Department of Anthropology and Archeology, School of Social Sciences, National University of Mongolia, Ulaanbaatar, Mongolia

  7. 7

    Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul 156-756, South Korea

  8. 8

    Department of Biology, Institute for Life Science, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, South Korea

  9. 9

    DNA Analysis Section, National Institute of Scientific Investigation, Seoul 158-707, South Korea

  10. 10

    Department of Archaelogy and Art History, College of Humanities, Donga University, Pusan 604-714, South Korea

  11. 11

    Division of Mathematical Science, College of Natural Sciences, Pukyong National University, Pusan 608-737, South Korea

  12. 12

    Department of Philosophy, College of Liberal Arts, Chung-Ang University, Seoul 156-756, South Korea

  13. 13

    Department of Life science, Chung-Ang University, Seoul 156-756, South Korea

*Department of Life Science and Department of Cultural Properties, Chung-Ang University, 221, Heukseok-dong, Dongjak-gu, Seoul 156-756, South Korea

Publication History

  1. Issue published online: 4 APR 2008
  2. Article first published online: 6 FEB 2008
  3. Manuscript Accepted: 19 NOV 2007
  4. Manuscript Received: 1 AUG 2007

Funded by

  • National Research Institute of Cultural Heritage of Cultural Heritage Administration (Conservation Technology Research and Development Project)

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Keywords:

  • DNA purification;
  • ancient bone;
  • PCR amplification;
  • ion-exchange columns;
  • PCR inhibitors

Abstract

A novel method of ancient DNA (aDNA) purification was developed using ion-exchange columns to improve PCR-amplifiable DNA extraction from ancient bone samples. Thirteen PCR-resistant ancient bone samples aged 500–3,300 years were tested to extract aDNA using a recently reported, silica-based aDNA extraction method and an ion-exchange column method for the further purification. The PCR success rates of the aDNA extracts were evaluated for the amplification ability of the fragments of mitochondrial DNA, a high-copy DNA, and amelogenin, a low-copy DNA. The results demonstrate that the further purification of silica-based aDNA extracts using ion-exchange columns considerably improved PCR amplification. We suggest that the ion-exchange column-based method will be useful for the improvement of PCR-amplifiable aDNA extraction, particularly from the poorly preserved, PCR-resistant, ancient samples. Am J Phys Anthropol, 2008. © 2008 Wiley-Liss, Inc.

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