Age estimation of archaeological remains using amino acid racemization in dental enamel: A comparison of morphological, biochemical, and known ages-at-death
Version of Record online: 8 APR 2009
Copyright © 2009 Wiley-Liss, Inc.
American Journal of Physical Anthropology
Volume 140, Issue 2, pages 244–252, October 2009
How to Cite
Griffin, R.C., Chamberlain, A.T., Hotz, G., Penkman, K.E.H. and Collins, M.J. (2009), Age estimation of archaeological remains using amino acid racemization in dental enamel: A comparison of morphological, biochemical, and known ages-at-death. Am. J. Phys. Anthropol., 140: 244–252. doi: 10.1002/ajpa.21058
- Issue online: 3 SEP 2009
- Version of Record online: 8 APR 2009
- Manuscript Accepted: 28 JAN 2009
- Manuscript Received: 24 SEP 2008
- Edinburgh Dental Institute
- U.K. Natural Environment Research Council. Grant Numbers: NER/B/S/1999/00007, NER/T/S/2002/00471
- amino acid composition
The poor accuracy of most current methods for estimating age-at-death in adult human skeletal remains is among the key problems facing palaeodemography. In forensic science, this problem has been solved for unburnt remains by the development of a chemical method for age estimation, using amino acid racemization in collagen extracted from dentine. Previous application of racemization methods to archaeological material has proven problematic. This study presents the application to archaeological human remains of a new age estimation method utilizing amino acid racemization in a potentially closed system—the dental enamel. The amino acid composition and extent of racemization in enamel from two Medieval cemeteries (Newcastle Blackgate and Grantham, England) and from a documented age-at-death sample from a 19th century cemetery (Spitalfriedhof St Johann, Switzerland) were determined. Alterations in the amino acid composition were detected in all populations, indicating that diagenetic change had taken place. However, in the Medieval populations, these changes did not appear to have substantially affected the relationship between racemization and age-at-death, with a strong relationship being retained between aspartic acid racemization and the morphological age estimates. In contrast, there was a poor relationship between racemization and age in the post-medieval documented age-at-death population from Switzerland. This appears to be due to leaching of amino acids post-mortem, indicating that enamel is not functioning as a perfectly closed system. Isolation of amino acids from a fraction of enamel which is less susceptible to leaching may improve the success of amino acid racemization for archaeological age estimation. Am J Phys Anthropol, 2009. © 2009 Wiley-Liss, Inc.