Age-at-death profiles obtained using the minimum number of individuals (MNI) for mass deposits of commingled human remains may be biased by over-representation of subadult individuals. A computer simulation designed in the R environment has shown that this effect may lead to misinterpretation of such samples even in cases where the completeness rate is relatively high. The simulation demonstrates that the use of the Most Likely Number of Individuals (MLNI) substantially reduces this bias. Am J Phys Anthropol 152:554–557, 2013. © 2013 Wiley Periodicals, Inc.