Potential conflict of interest: None provided.
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Original Article
Methylglyoxal: (active agent of manuka honey) in vitro activity against bacterial biofilms†
Article first published online: 25 MAY 2011
DOI: 10.1002/alr.20073
Copyright © 2011 American Rhinologic Society-American Academy of Otolaryngic Allergy, LLC
Issue
International Forum of Allergy & Rhinology
Volume 1, Issue 5, pages 348–350, September/October 2011
Additional Information
How to Cite
Kilty, S. J., Duval, M., Chan, F. T., Ferris, W. and Slinger, R. (2011), Methylglyoxal: (active agent of manuka honey) in vitro activity against bacterial biofilms. International Forum of Allergy & Rhinology, 1: 348–350. doi: 10.1002/alr.20073
- †
Publication History
- Issue published online: 3 OCT 2011
- Article first published online: 25 MAY 2011
- Manuscript Accepted: 1 MAY 2011
- Manuscript Revised: 7 APR 2011
- Manuscript Received: 22 FEB 2011
- Abstract
- Article
- References
- Cited By
Keywords:
- bacteria;
- biofilm;
- manuka honey;
- methylglyoxal;
- sinusitis
Abstract
Background
Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA) biofilms are associated with poor chronic rhinosinusitis (CRS) disease control following surgery. Manuka honey (MH) has been shown to be both an effective in vitro treatment agent for SA and PA biofilms and nontoxic to sinonasal respiratory mucosa. Methylglyoxal (MGO) has been reported to be the major antibacterial agent in MH. The effect of this agent against SA and PA biofilms has yet to be reported. Our objective was to determine the in vitro effect of MGO against biofilms of SA and PA, via in vitro testing of MGO against bacterial biofilms.
Methods
An established biofilm model was used to determine the effective concentration (EC) of MGO against 10 isolates of methicillin-resistant SA (MRSA) and PA. The EC of MGO was also determined against planktonic (free-swimming) MRSA and PA.
Results
For MRSA, the EC against planktonic organisms was a concentration of 0.08 mg/mL to 0.3 mg/mL whereas against the biofilm MRSA isolates, the EC ranged from 0.5 mg/mL to 3.6 mg/mL. For PA, the EC against planktonic organisms was a concentration of 0.15 mg/mL to 1.2 mg/mL for planktonic organisms whereas against the biofilm PA isolates, the EC ranged from 1.8 mg/mL to 7.3 mg/mL.
Conclusion
MGO, a component of MH, is an effective antimicrobial agent against both planktonic and biofilm MRSA and PA organisms in vitro.