Potential conflict of interest: None provided.
Upregulation of Bcl-2 in nasal polyps from patients with cystic fibrosis
Version of Record online: 7 NOV 2012
© 2013 ARS-AAOA, LLC
International Forum of Allergy & Rhinology
Volume 3, Issue 3, pages 199–203, March 2013
How to Cite
How to Cite this Article: Upregulation of Bcl-2 in nasal polyps from patients with cystic fibrosis. Int Forum Allergy Rhinol, 2013; 3:199–203., ,
- Issue online: 12 MAR 2013
- Version of Record online: 7 NOV 2012
- Manuscript Accepted: 21 AUG 2012
- Manuscript Revised: 5 AUG 2012
- Manuscript Received: 18 APR 2012
- nasal polyps;
- chronic rhinosinusitis;
- cystic fibrosis;
- protein array
Nasal polyps in patients with cystic fibrosis (CF) are believed to be phenotypically different than polyps affecting non-CF patients. The aim of this study was to investigate differences in cell cycle regulatory mechanisms between these 2 groups. In this prospective study at a tertiary care academic medical center, multiple techniques were used to confirm the upregulation of antiapoptotic Bcl-2 family proteins in CF polyps.
Nasal polyps were prospectively obtained from CF and non-CF patients. The Sigma Panorama Protein Microarray for Cell Signaling was used to identify differences in protein expression between the 2 polyp groups. Western blot analysis confirmed altered expression of a subset of these proteins. Immunohistochemical staining was performed on archived tissue to further investigate B-cell lymphoma 2 protein (Bcl-2) expression. Following review by a pathologist, slides were digitized using an Aperio™ ScanScope XT system and staining intensity was quantified with the Positive Pixel Count algorithm. The mean staining intensity for each polyp group was compared.
The protein microarray suggested a greater than 2-fold upregulation of Bcl-xl in CF polyps relative to non-CF polyps. Western blot analysis confirmed the upregulation in CF polyps of Bcl-2, a more commonly studied protein analog of Bcl-xl. The CF polyp group was noted to have a higher quantitative intensity of immunohistochemical staining for Bcl-2 compared to the non-CF group (p < 0.05).
Through multiple modalities of protein investigation, we have demonstrated an upregulation of Bcl-2 family proteins in CF polyps relative to polyps from non-CF patients.