Potential conflict of interest: W.R.R. is on the Speaker's Bureau for Thermo Fisher Scientific, who provided the microarray testing at no charge, but played no other role in this study.
Detecting local immunoglobulin E from mucosal brush biopsy of the inferior turbinates using microarray analysis
Article first published online: 7 NOV 2012
© 2013 ARS-AAOA, LLC
International Forum of Allergy & Rhinology
Volume 3, Issue 5, pages 399–403, May 2013
How to Cite
How to Cite this Article: Detecting local immunoglobulin E from mucosal brush biopsy of the inferior turbinates using microarray analysis. Int Forum Allergy Rhinol, 2013; 3:399–403.
Presented at the American Academy of Otolaryngic Allergy Annual Meeting on September 10, 2012, Washington, DC.
- Issue published online: 17 MAY 2013
- Article first published online: 7 NOV 2012
- Manuscript Accepted: 25 SEP 2012
- Manuscript Revised: 18 SEP 2012
- Manuscript Received: 27 JUL 2012
- mucosal brush biopsy;
- microarray analysis;
- allergic rhinitis;
- inferior turbinate;
- local IgE
It has been previously demonstrated that local, antigen-specific immunoglobulin E (IgE) can be detected using a standard in vitro assay of lysed epithelial cells in saline, harvested via nasal mucosal brush biopsy (MBB). However, compared to surgical biopsy or serum, smaller amounts of IgE are harvested using MBB, making detection much more difficult. Microarray analysis (MA) requires less IgE for detection, making this an attractive option for MBB. The goals of this study were to compare MA to a standard IgE assay for detecting antigen-specific IgE from MBB and to test the association between the presence of multiple positive components on MA with specific IgE on standard assay and skin-prick testing (SPT) grade.
MBB samples from 18 allergic rhinitis patients, which were previously tested for antigen-specific IgE to common airborne allergens using a standard IgE assay, underwent MA for antigen-specific IgE to multiple components of airborne and food allergens. Fisher's exact probability testing was used to measure the strength of association between the 2 testing modalities for Timothy grass, ragweed, cat, Alternaria, and D. farinae.
MA correlated very highly with standard assay (p < 0.0001) and 50% of positive antigens on MA detected multiple components to that antigen. The presence of multiple components was not associated with specific IgE levels on standard assay or SPT grade.
This is the first demonstration that antigen-specific IgE in saline samples can be measured using MA. The ability of MA to measure smaller amounts of IgE, with similar accuracy, may give it a potential advantage for MBB analysis in the future.