Characterization of B-cell subpopulations in patients with chronic rhinosinusitis

Authors

  • Alkis J. Psaltis MD, PhD,

    Corresponding author
    1. Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, SC
    • Correspondence to: Alkis Psaltis, MD, PhD, Department of Otolaryngology–Head and Neck Surgery, Stanford School of Medicine, Stanford University, 801 Welch Road, Palo Alto, CA 94303; e-mail: alkispsaltis@gmail.com

    Search for more papers by this author
  • Rodney J. Schlosser MD,

    1. Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, SC
    2. Ralph H. Johnson VA Medical Center, Charleston, SC
    Search for more papers by this author
  • James R. Yawn BS,

    1. Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, SC
    Search for more papers by this author
  • Oswaldo Henriquez MD,

    1. Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, SC
    Search for more papers by this author
  • Jennifer K. Mulligan PhD

    1. Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, SC
    2. Ralph H. Johnson VA Medical Center, Charleston, SC
    3. Department of Pediatrics, Medical University of South Carolina, Charleston, SC
    Search for more papers by this author

  • Funding sources for the study: American Academy of Otolaryngic Allergy (to A.J.P.); Garnett Passe and Rodney Williams Memorial Foundation (to A.J.P.); Department of Veterans Affairs (to R.J.S.); Flight Attendant Medical Research Institutes (to J.K.M. and R.J.S.).

  • Potential conflict of interest: None provided.

Abstract

Background

Recent research suggest that B and plasma cells may play an important role in the pathogenesis of chronic rhinosinusitis with nasal polyposis (CRSwNP). The purpose of this study was to subcharacterize the B cell response in the sinus mucosa of control and CRS patients.

Methods

Representative tissue samples and peripheral blood samples were obtained from controls, CRS without nasal polyps (CRSsNP) and CRSwNP. Using single-cell suspension flow cytometry these samples were analyzed for overall and stage-specific B and plasma cell percentages.

Results

Both atopic and nonatopic CRSwNP patients showed an increase in local numbers of naive, active, and memory B cells compared to controls. CRSsNP patients only showed local elevations of naive B cells. Plasma cells were only significantly elevated in the sinus tissue of atopic CRSwNP patients. These local tissue increases did not correlate with increased numbers of circulating B cells.

Conclusion

This study provides further evidence of an important role of B cells in CRSwNP patients. The local increase appears to be independent of a systemic response.

Ancillary