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Nasal polyp chitinolytic activity associated with smoking or allergy

Authors

  • Kyung Wook Heo MD, PhD,

    1. Department of Otorhinolaryngology–Head and Neck Surgery, Inje University, College of Medicine, Busan Paik Hospital, Busan, Korea
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  • Tae Yong Kim MD,

    1. Department of Otorhinolaryngology–Head and Neck Surgery, Inje University, College of Medicine, Busan Paik Hospital, Busan, Korea
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  • Seong Kook Park MD, PhD,

    Corresponding author
    1. Department of Otorhinolaryngology–Head and Neck Surgery, Inje University, College of Medicine, Busan Paik Hospital, Busan, Korea
    • Correspondence to: Seong Kook Park, MD, PhD, Department of Otorhinolaryngology–Head and Neck Surgery, Inje University Busan Paik Hospital #633-165, Gaegeum-dong, Busanjin-Ku, Busan, 614-735, Korea; e-mail: sinus4@paik.ac.kr

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  • Ga Bin Park PhD,

    1. Department of Anatomy and Research Center for Tumor Immunology, Inje University, College of Medicine, Busan Paik Hospital
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  • Dae Young Hur MD, PhD,

    1. Department of Anatomy and Research Center for Tumor Immunology, Inje University, College of Medicine, Busan Paik Hospital
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  • Woo Yong Bae MD, PhD

    1. Department of Otorhinolaryngology–Head and Neck Surgery, Dong-A University, College of Medicine, Busan, Korea
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  • Funding sources for the study: Inje University research grant (2013).

  • Potential conflict of interest: None provided.

Abstract

Background

Cells of the innate immune system that are implicated in allergy and immunity bind to chitin during tissue infiltration in a process negatively regulated by vertebrate chitinases. Both acidic mammalian chitinase (AMCase) and chitotriosidase (ChT) exert chitinolytic activity. The levels of activities of these enzymes in nasal polyps (NPs) of subjects who smoke or who suffer from allergies are unknown. In the present work, we measured the activities of AMCase and ChT in NPs of smokers and allergic subjects.

Methods

We report a prospective cohort study in a tertiary care facility. AMCase and ChT activities of NPs were measured in buffers of several pH values using the fluorogenic substrate 4-methylumbelliferyl-β-d-N,N,N″-triacetyl-chitotriose.

Results

The activities of AMCase and ChT in NPs did not differ significantly among smokers, nonsmokers, and ex-smokers. AMCase and ChT activities were significantly higher in NPs of allergic subjects than in NPs of those who did not suffer from allergy.

Conclusion

Increased levels of chitinolytic activities in NPs were associated with the allergic rhinitis. We suggest that control of such rhinitis may help to prevent the development and growth of NPs.

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