The kinetics of charges in dry bacteriorhodopsin Langmuir–Blodgett films—an analysis and comparison of electrical and optical signals

Authors

  • Marjo Ikonen,

    1. Department of Chemistry, Physical Chemistry Division, University of Helsinki, PO Box 13 (Meritullinkatu 1C), FIN-00014 Helsinki, Finland
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  • Alexey Yu. Sharonov,

    1. Department of Chemistry, Physical Chemistry Division, University of Helsinki, PO Box 13 (Meritullinkatu 1C), FIN-00014 Helsinki, Finland
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    • General Physics Institute, Vavilov St. 38, 117942 Moscow, Russian Federation.

  • Nikolai V. Tkachenko,

    1. Department of Chemistry, Physical Chemistry Division, University of Helsinki, PO Box 13 (Meritullinkatu 1C), FIN-00014 Helsinki, Finland
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    • General Physics Institute, Vavilov St. 38, 117942 Moscow, Russian Federation.

  • Helge Lemmetyinen

    1. Department of Chemistry, Physical Chemistry Division, University of Helsinki, PO Box 13 (Meritullinkatu 1C), FIN-00014 Helsinki, Finland
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Abstract

The decay of the M intermediate and the recovery kinetics of bacteriorhodopsin (bR) were detected optically in dry and wet bR Langmuir–Blodgett (LB) films and electrically in dry films. From the electrical signals the kinetics of the proton motion was distinguished from the cell function and it was found to be equal to that determined optically for the M intermediate decay. The rate-determining step for the recovery rate of bR was found to be the M state.

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