This article is a US Government work and, as such, is in the public domain in the United States of America.
Lineage pathway of human brain progenitor cells identified by JC virus susceptability†
Article first published online: 28 MAR 2003
Published 2003 Wiley-Liss, Inc.
Annals of Neurology
Volume 53, Issue 5, pages 636–646, May 2003
How to Cite
Messam, C. A., Hou, J., Gronostajski, R. M. and Major, E. O. (2003), Lineage pathway of human brain progenitor cells identified by JC virus susceptability. Ann Neurol., 53: 636–646. doi: 10.1002/ana.10523
- Issue published online: 25 APR 2003
- Article first published online: 28 MAR 2003
- Manuscript Accepted: 19 DEC 2002
- Manuscript Revised: 18 DEC 2002
- Manuscript Received: 14 JUN 2002
- NIH. Grant Numbers: HD34908, DK58401
Multipotential human central nervous system progenitor cells, isolated from human fetal brain tissue by selective growth conditions, were cultured as undifferentiated, attached cell layers. Selective differentiation yielded highly purified populations of neurons or astrocytes. This report describes the novel use of this cell culture model to study cell type–specific recognition of a human neurotropic virus, JC virus. Infection by either JC virions or a plasmid encoding the JC genome demonstrated susceptibility in astrocytes and, to a lesser degree, progenitor cells, whereas neurons remained nonpermissive. JC virus susceptibility correlated with significantly higher expression of the NFI-X transcription factor in astrocytes than in neurons. Furthermore, transfection of an NFI-X expression vector into progenitor-derived neuronal cells before infection resulted in viral protein production. These results indicate that susceptibility to JC virus infection occurs at the molecular level and also suggest that differential recognition of the viral promoter sequences can predict lineage pathways of multipotential progenitor cells in the human central nervous system. Ann Neurol 2003