Cystatin C in cerebrospinal fluid and multiple sclerosis

Authors

  • Sara F. Hansson MSc,

    1. Department of Clinical Neuroscience, University of Göteborg, Sahlgrenska University Hospital/Mölndal, Mölndal, Sweden
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  • Anja Hviid Simonsen MSc,

    1. Memory Disorders Research Group, Department of Neurology, Copenhagen University Hospital, Copenhagen, Denmark
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  • Henrik Zetterberg MD, PhD,

    1. Department of Clinical Neuroscience, University of Göteborg, Sahlgrenska University Hospital/Mölndal, Mölndal, Sweden
    2. Department of Clinical Chemistry and Transfusion Medicine, University of Göteborg, Sahlgrenska University Hospital, Göteborg, Sweden
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  • Oluf Andersen MD, PhD,

    1. Department of Neurology, University of Göteborg, Sahlgrenska University Hospital, Göteborg, Sweden
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  • Sara Haghighi MD, PhD,

    1. Department of Neurology, University of Göteborg, Sahlgrenska University Hospital, Göteborg, Sweden
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  • Inger Fagerberg BSc,

    1. Department of Clinical Chemistry and Transfusion Medicine, University of Göteborg, Sahlgrenska University Hospital, Göteborg, Sweden
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  • Ulf Andréasson PhD,

    1. Department of Clinical Neuroscience, University of Göteborg, Sahlgrenska University Hospital/Mölndal, Mölndal, Sweden
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  • Ann Westman-Brinkmalm PhD,

    1. Department of Clinical Neuroscience, University of Göteborg, Sahlgrenska University Hospital/Mölndal, Mölndal, Sweden
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  • Anders Wallin MD, PhD,

    1. Department of Clinical Neuroscience, University of Göteborg, Sahlgrenska University Hospital/Mölndal, Mölndal, Sweden
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  • Ulla Rüetschi PhD,

    1. Department of Clinical Chemistry and Transfusion Medicine, University of Göteborg, Sahlgrenska University Hospital, Göteborg, Sweden
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  • Kaj Blennow MD, PhD

    Corresponding author
    1. Department of Clinical Neuroscience, University of Göteborg, Sahlgrenska University Hospital/Mölndal, Mölndal, Sweden
    2. Department of Clinical Chemistry and Transfusion Medicine, University of Göteborg, Sahlgrenska University Hospital, Göteborg, Sweden
    • Department of Clinical Neuroscience, University of Göteborg, Sahlgrenska University Hospital/Mölndal, S-431 80 Mölndal, Sweden
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Abstract

Objective

A recent study using surface-enhanced laser desorption/ionization time-of-flight analysis of cerebrospinal fluid identified a 12.5kDa truncated isoform of cystatin C (CysC) as a specific biomarker for multiple sclerosis (MS).

Methods

Surface-enhanced laser desorption/ionization time-of-flight analysis of cerebrospinal fluid samples from 43 MS patients and 46 healthy control subjects.

Results

Full-length CysC (13.4kDa) concentration was similar in MS and control samples. The 12.5kDa CysC protein was produced from full-length CysC by N-terminal cleavage during storage at −20°C.

Interpretation

The 12.5kDa CysC isoform is a storage-related artifact and is not useful as a diagnostic marker for MS. Ann Neurol 2007;62:193–196

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