Additional Supporting Information can be found in the online version of this article.

ANA_22034_sm_SupFigS1.tif10351KFig. S1. Intact dopaminergic nigrostriatal system in Hq mutant mice. Stereological cell counts of SNpc TH-immunorective neurons (left panel) and optical densitometry of striatal TH immunoreactivity (right panel) from saline- and MPTP-intoxicated six and nine month-old WT or Hq mice. While no statistically significant differences were observed between the different groups of animals, 9 month-old mice exhibited a non-significant trend towards decreased striatal TH values compared to WT mice [ANOVA, (F3,11)=1,116; p=0,398; Student t-test (p=0,134)].
ANA_22034_sm_SupFigS2.tif4926KFig. S2. Dopaminergic VTA neurons from Hq mice are more susceptible to MPTP neurotoxicity. Stereological cell counts of VTA TH-immunorective neurons from saline- and MPTP-intoxicated six month-old WT or Hq mice. Top panels display representative micrographs of TH-immunostained (brown) thionin-counterstained (blue) mesencephalon from the different groups of animals. SNpc, substantia nigra pars compacta. VTA, ventral tegmental area. *p<0.05, compared to saline-injected WT and Hq mice and to MPTP-injected wild-type mice [Fgenotype(1,11)=5,88, Ftreatment(1,11)=34,58, and Fgenotype × treatment(1,11)=14,37]. Scale bar=500 μm.
ANA_22034_sm_SupFigS3.tif9511KFig. S3. Tempol does not protect striatal dopaminergic terminals of Hq mice against MPTP intoxication. Optical densitometry of striatal TH immunoreactivity of saline- or MPTP-injected WT or Hq mice, treated or not with tempol. *p<0.05, compared to saline-injected animals.

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