Potential Conflicts of Interest Nothing to report.
Human prion strain selection in transgenic mice†
Version of Record online: 2 AUG 2010
Copyright © 2010 American Neurological Association
Annals of Neurology
Volume 68, Issue 2, pages 151–161, August 2010
How to Cite
Giles, K., Glidden, D. V., Patel, S., Korth, C., Groth, D., Lemus, A., DeArmond, S. J. and Prusiner, S. B. (2010), Human prion strain selection in transgenic mice. Ann Neurol., 68: 151–161. doi: 10.1002/ana.22104
- Issue online: 2 AUG 2010
- Version of Record online: 2 AUG 2010
- Manuscript Accepted: 20 MAY 2010
- Manuscript Revised: 19 MAY 2010
- Manuscript Received: 30 APR 2010
- NIA, NIH. Grant Numbers: AG02132, AG10770, AG021601, AG031220, AI064709, NS041997
- Fight for Mike Homer Program
- Sherman Fairchild, Leila Y. and Harold G. Mathers Charitable, Hillblom, and Lincy Foundations
Transgenic (Tg) mice expressing chimeras of mouse and human prion proteins (PrPs) have shorter incubation periods for Creutzfeldt-Jakob disease (CJD) prions than mice expressing full-length human PrP. Increasing the sequence similarity of the chimeric PrP to mouse PrP, by reverting human residues to mouse, resulted in a Tg line, denoted Tg22372, which was susceptible to sporadic (s) CJD prions in ∼110 days.
Mice expressing chimeric mouse/human PrP transgenes were produced. The mice were inoculated intracerebrally with extracts prepared from the brains of patients who died of CJD. Onset of neurological dysfunction marked the end of the incubation time. After sacrifice of the Tg mice, their brains were analyzed for PrPSc and neuropathological changes.
Reversion of 1 additional residue (M111V) resulted in a new Tg line, termed Tg1014, susceptible to sCJD prions in ∼75 days. Tg1014 mice also have shorter incubation periods for variant (v) CJD prions, providing a more tractable model for studying this prion strain. Transmission of vCJD prions to Tg1014 mice resulted in 2 different strains, determined by neuropathology and biochemical analysis, which correlated with the length of the incubation time. One strain had the biochemical, neuropathological, and transmission characteristics, including longer incubation times, of the inoculated vCJD strain; the second strain produced a phenotype resembling that of sCJD prions including relatively shorter incubation periods. Mice with intermediate incubation periods for vCJD prions had a mixture of the 2 strains. Both strains were serially transmitted in Tg1014 mice, which led to further reduction in incubation periods. Conversion of vCJD-like to sCJD-like strains was favored in Tg1014 mice more than in the Tg22372 line. The single amino acid difference therefore appears to offer selective pressure for propagation of the sCJD-like strain.
These 2 Tg mouse lines provide relatively rapid models to study human prion diseases as well as the evolution of human prion strains. ANN NEUROL 2010;68:151–161