Additional Supporting Information can be found in the online version of this article.

ANA_22162_sm_suppinfo_fig1.doc2176KSupporting Figure 1: PCR products for EAAC1 genotyping. EAAC1−/− mice are identified by a 1295 bp band containing a sequence unique to the neomycin resistance cassette. Wild-type (EAAC1+/+) mice are identified by a 63 bp band containing a sequence unique to the EAAC1 codon. Heterozygotes show both bands (not shown). The band at 325 bp is a non-specific product unrelated to genotype.
ANA_22162_sm_suppinfo_fig2.doc1429KSupporting Figure 2: Criteria for grading resting and activated microglia. The microglia exemplifying the grading criteria are immunostained for CD11b and were identified in midbrain of EAAC1−/− mice. Microglia with either hypertrophic or amoeboid morphology are considered “activated”.
ANA_22162_sm_suppinfo_fig3.doc40KSupporting Figure 3: Biogenic amines in striatum of wild-type and EAAC1−/− mice. Biogenic amines were analyzed in mice aged 9-11 months, n = 5 of each genotype. DA, dopamine; DOPAC, 3,4-dihydroxyphenylacetic acid; 3-MT, 3-methoxytyramine; HVA, homovanillic acid; 5-HT, serotonin; 5-HIAA, 5-hydroxyindoleacetic acid; NE, norepinephrine.

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