Ari E. Berman and Wai Yee Chan contributed equally to this work.
N-acetylcysteine prevents loss of dopaminergic neurons in the EAAC1−/− mouse
Article first published online: 23 NOV 2010
Copyright © 2010 American Neurological Association
Annals of Neurology
Volume 69, Issue 3, pages 509–520, March 2011
How to Cite
Berman, A. E., Chan, W. Y., Brennan, A. M., Reyes, R. C., Adler, B. L., Suh, S. W., Kauppinen, T. M., Edling, Y. and Swanson, R. A. (2011), N-acetylcysteine prevents loss of dopaminergic neurons in the EAAC1−/− mouse. Ann Neurol., 69: 509–520. doi: 10.1002/ana.22162
- Issue published online: 28 MAR 2011
- Article first published online: 23 NOV 2010
- Manuscript Accepted: 13 JUL 2010
- Manuscript Revised: 3 JUL 2010
- Manuscript Received: 26 JUL 2009
- U.S. Department of Veterans Affairs
- Michael J. Fox Foundation for Parkinson's Research
Additional Supporting Information can be found in the online version of this article.
|ANA_22162_sm_suppinfo_fig1.doc||2176K||Supporting Figure 1: PCR products for EAAC1 genotyping. EAAC1−/− mice are identified by a 1295 bp band containing a sequence unique to the neomycin resistance cassette. Wild-type (EAAC1+/+) mice are identified by a 63 bp band containing a sequence unique to the EAAC1 codon. Heterozygotes show both bands (not shown). The band at 325 bp is a non-specific product unrelated to genotype.|
|ANA_22162_sm_suppinfo_fig2.doc||1429K||Supporting Figure 2: Criteria for grading resting and activated microglia. The microglia exemplifying the grading criteria are immunostained for CD11b and were identified in midbrain of EAAC1−/− mice. Microglia with either hypertrophic or amoeboid morphology are considered “activated”.|
|ANA_22162_sm_suppinfo_fig3.doc||40K||Supporting Figure 3: Biogenic amines in striatum of wild-type and EAAC1−/− mice. Biogenic amines were analyzed in mice aged 9-11 months, n = 5 of each genotype. DA, dopamine; DOPAC, 3,4-dihydroxyphenylacetic acid; 3-MT, 3-methoxytyramine; HVA, homovanillic acid; 5-HT, serotonin; 5-HIAA, 5-hydroxyindoleacetic acid; NE, norepinephrine.|
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