Additional Supporting Information can be found in the online version of this article.

ANA_22281_sm_SuppFig1.tif866KSupporting Information Figure 1. Effect of chronic cerebral hypoperfusion on CSD susceptibility. Representative 30-minute tracings showing repetitive CSDs triggered during continuous topical KCl (300mM) application in sham-operated or bilateral carotid stenosis mice. Chronic cerebral hypoperfusion was induced by placing steel coils around common carotid arteries 9 weeks before testing. Topical KCl application evoked fewer CSDs after bilateral carotid stenosis compared to sham-operated controls. CSD durations also tended to be prolonged after carotid stenosis (please see the Results section and Table 1 for quantitative analysis). Calibration bars: 10 mV, 2 min.
ANA_22281_sm_SuppFig2.tif985KSupporting Information Figure 2. CSD-induced blood flow changes did not differ between TgNotch3R90C and WT mice. A representative tracing showing blood flow changes during two consecutive CSDs evoked by epidural application of 300mM KCl 15 minutes apart (upper panel), and averaged blood flow data from TgNotch3R90C and WT mice (lower panel) during the first (left) and second CSD (right). Cortical blood flow changes were measured using laser speckle flowmetry non-invasively through intact skull. Inset shows the position of laser speckle imaging field and the region of interest (ROI) used to quantify the blood flow changes, as previously described26. Both the magnitude and the latency of blood flow deflection points were measured and plotted as a function of time after CSD onset to reconstruct the blood flow transients in each group for statistical comparison. Blood flow changes during CSD were similar to previous reports26 and did not differ between the TgNotch3R90C and WT groups. Please also see the Supporting Movie. N=6 each. Data are mean ± standard deviation.

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