Additional supporting information can be found in the online version of this article.

ANA_22363_sm_suppinfofigure1.mpg2168KSupplementary Figure 1. Bar-cross test of an 18 months old Abcd1-/Abcd2-/- mouse, fed for 6 months with normal chow as described.
ANA_22363_sm_suppinfofigure2.mpg1376KSupplementary Figure 2. Bar-cross test of an 18 months old Abcd1-/Abcd2-/- mouse fed for 6 months with antioxidants as described.
ANA_22363_sm_suppinfofigure3.doc506KSupplementary Figure 3. Oxidative stress, myelin and axonal pathologies in 18-month-old Abcd1-/Abcd2-/- spinal cord are rescued by antioxidant cocktail. Longitudinal sections of the dorsal spinal cord in Wt (A, D, G, J, M, P), Abcd1-/Abcd2-/- (B, E, H, K, N, Q) and Abcd1-/Abcd2-/- + Antx (C, F, I, L, O, R) mice processed for 8-oxodG (A-C), lectin Lycopericon esculentum (D-F), GFAP (G-I), synaptophysin (J-L), APP (M-O) and Sudan black (P-R). Bar=25μm. S) Quantification of APP and synaptophysin accumulation in axonal swellings in Wt, Abcd1-/Abcd2-/- and Abcd1-/Abcd2-/- + Antx mice. Significant differences were determined as described in materials and methods (n=5-6 mice per genotype and condition; *P<0.05, **P<0.01, ***P<0.001).
ANA_22363_sm_suppinfo.doc45KSupporting Information
ANA_22363_sm_suppinfotable.doc29KSupplementary Table. Summary of the main pathological findings in 1 cm long longitudinal section of the dorsal spinal cord in Wt , Abcd1-, Abcd1- + Antx mice at 22m of age and Wt, Abcd1-/Abcd2−/− , Abcd1-/Abcd2−/− + Antx mice at 18m of age (n=5-6 mice per genotype and condition). DNA damage is stained with 8-oxodG, microglial cells with lectin Lycopericon esculentum and astrocytes with GFAP. *, Normal appearance; **, slight and ***, marked increase in DNA damage or in the number and size of astrocytes and microglia. Sudan black visualizes abnormal lipidic droplets resulting from myelin debris.

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