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Additional Supporting Information can be found in the online version of this article.

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ANA_22665_sm_SuppFig1.tif1626KSupplementary Figure 1. Confirmation of impaired coordinated motor behaviors in a second, independently derived line of L7-1.8TG mice excludes insertion-site effects. Black bars, L7-1.8TG mice; white bars, WT littermates. A. Transgenic mice perform poorly in a wire hang test (p=0.0015, n= 20 WT, 18 TG). B. Transgenic mice fall with shorter latency from a constant speed rotarod (p=0.0287, n=18 WT, 10 TG). C. Grip strength is similar for wildtype and transgenic mice (p=0.2004, n= 13 WT, 9 TG). D. Response to heat stimuli in the hindpaw radiant heat (Hargreaves) test is similar for wildtype and transgenic mice (p=0.9432, n=8 WT, 8 TG). E. Response to mechanical stimuli in the von Frey test is similar for wildtype and transgenic mice (p=0.561, n=8 WT, 8 TG).
ANA_22665_sm_SuppFig2.tif9259KSupplementary Figure 2. Selective upregulation of Nav1.8 in Purkinje neurons and not in other regions of the central nervous system. A. Full sagittal brain section from a wildtype mouse with EAE, showing in situ hybridization signal for Nav1.8. Note that the only specific labeling is present in the Purkinje cell layer of the cerebellum (arrowheads). Inset, higher magnification view of boxed region. B. Full sagittal brain section from a Nav1.8KO mouse with EAE, showing in situ hybridization signal for Nav1.8. No labeling is present anywhere in the brain, although shadowing in the white matter tracts is similar to wildtype. Asterisk denotes a fold in the tissue. Inset, higher magnification view of boxed region. C. Spinal cord section from a Nav1.8-Cre-reporter mouse without EAE. Note that, although dense fluorescent reporter labeling is present in primary afferent terminals in the dorsal horn, no intrinsic spinal cord neurons express Nav1.8. D. Spinal cord section from a Nav1.8-Cre-reporter mouse on Day 15 after induction of EAE. Primary afferent terminals are labeled as in the control mouse, and no new expression of Nav1.8 is induced in any spinal cord region.
ANA_22665_sm_SuppFig3.tif2590KSupplementary Figure 3. Immune cell infiltration is similar in WT and Nav1.8KO mice with EAE. A. Top panel, No CD45-positive cells were found in lumbar spinal cord samples from control mice without EAE. Middle panel, CD45-positive immune cell infiltration was prominent in spinal cord samples from WT mice on day 10 after induction of EAE. Bottom panel, CD45-positive immune cell infiltration was prominent in spinal cord samples from Nav1.8KO mice at the same stage of EAE. B. CD45-positive immune cell infiltration was similar in WT and Nav1.8KO mice (p>0.05, n=4/group).

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