Early detection of thrombin activity in neuroinflammatory disease
Article first published online: 24 FEB 2014
© 2014 The Authors Annals of Neurology published by Wiley Periodicals, Inc. on behalf of Child Neurology Society/American Neurological Association.
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Annals of Neurology
Volume 75, Issue 2, pages 303–308, February 2014
How to Cite
Davalos, D., Baeten, K. M., Whitney, M. A., Mullins, E. S., Friedman, B., Olson, E. S., Ryu, J. K., Smirnoff, D. S., Petersen, M. A., Bedard, C., Degen, J. L., Tsien, R. Y. and Akassoglou, K. (2014), Early detection of thrombin activity in neuroinflammatory disease. Ann Neurol., 75: 303–308. doi: 10.1002/ana.24078
- Issue published online: 19 MAR 2014
- Article first published online: 24 FEB 2014
- Accepted manuscript online: 29 NOV 2013 03:48AM EST
- Manuscript Accepted: 23 NOV 2013
- Manuscript Revised: 7 NOV 2013
- Manuscript Received: 20 SEP 2013
Although multiple sclerosis (MS) has been associated with the coagulation system, the temporal and spatial regulation of coagulation activity in neuroinflammatory lesions is unknown. Using a novel molecular probe, we characterized the activity pattern of thrombin, the central protease of the coagulation cascade, in experimental autoimmune encephalomyelitis. Thrombin activity preceded onset of neurological signs, increased at disease peak, and correlated with fibrin deposition, microglial activation, demyelination, axonal damage, and clinical severity. Mice with a genetic deficit in prothrombin confirmed the specificity of the thrombin probe. Thrombin activity might be exploited for developing sensitive probes for preclinical detection and monitoring of neuroinflammation and MS progression. Ann Neurol 2014;75:303–308