Chronic experimental allergic encephalomyelitis produced by bovine proteolipid apoprotein: Immunological studies in rabbits

Authors

  • Dr Franca Cambi MD,

    Corresponding author
    1. Biochemistry Department, Eunice Kennedy Shriver Center for Mental Retardation, Maltham, MA 02254
    2. Department of Neurology, Harvard Medical School, Boston, MA 02115
    • E. K. Shiver Center, 200 Tarpelo Rd, Waltham, MA 02254
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  • Marjorie B. Lees PhD,

    1. Biochemistry Department, Eunice Kennedy Shriver Center for Mental Retardation, Maltham, MA 02254
    2. Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115
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  • Rosemarie M. Williams DVM,

    1. Department of Pathology, Tufts University School of Veterinary Medicine, Boston, MA 021 11
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  • Wendy B. Macklin PhD

    1. Biochemistry Department, Eunice Kennedy Shriver Center for Mental Retardation, Maltham, MA 02254
    2. Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115
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Abstract

A chronic experimental allergic encephalomyelitis (EAE) has been produced in rabbits sensitized with bovine white matter proteolipid apoprotein. Eleven of 12 animals developed clinical disease one to six months after immunization with a single dose of the apoprotein. The clinical course was characterized by posterior ataxia, flaccid paralysis progressing to spastic paralysis, and incontinence. Spontaneous relapses and remissions were observed in 3 rabbits. Histologically, acute and chronic encephalomyelitis accompanied by primary demyelination were observed. Serumantibody production, assayed by both an enzyme-linked immunosorbent assay and an electroblot procedure, did not correlate with either the clinical course or the histopathological findings. Delayed hypersensitivity to proteolipid apoprotein was observed in all rabbits prior to the onset of clinical signs. The data suggest that lymphocytes specifically sensitized to the proteolipid may be involved in the pathogenesis of the demyelination in chronic EAE.

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