In vivo PET Imaging in rat of dopamine terminals reveals functional neural transplants

Authors

  • Anna-Liisa Brownell PhD,

    1. Neuroregenration Laboratory, McLean Hospital, Harvard Medical School, Belmont, MA
    2. Departments of Radiology, Harvard Medical School, Boston, MA
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  • Eli Livni PhD,

    1. Departments of Radiology, Harvard Medical School, Boston, MA
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  • Wendy Galpern PhD,

    1. Neuroregenration Laboratory, McLean Hospital, Harvard Medical School, Belmont, MA
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  • Dr Ole Isacson DrMedSc

    Corresponding author
    1. Departments of Radiology, Harvard Medical School, Boston, MA
    2. Neurology, Massachusetts General Hospital, Boston, MA
    3. Program in Neuroscience, Harvard Medical School, Boston, MA
    • Neuroregeneration Laboratory, Harvard Medical School, McLean Hospital, MRC 119, 115 Mill Street, Belmont, MA
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Abstract

Positron emission tomography (PET) and carbon-11-labeled 2B-carbomethoxy-3B-(4-fluorophenyl)tropane (11C-CFT or 11-WIN 35,428) were used as molecular markers for striatal presynaptic dopamine (DA) transporters in a unilateral Parkinson's disease rat neurotransplantation model. In the lesioned striatum, the binding ratio measured by the DA presynaptic marker was reduced to 15% to 35% of the intact side (or unoperated control). After grafting with non-DA cells (from dorsal mesencephalon), the DA binding ratio remained reduced to levels observed before transplantation and rats showed no behavioral recovery. In contrast, after DA neuronal transplantation, behavioral recovery occurred only after the 11C-CFT binding ratio had increased to 75% to 85% of the intact side. This study provides direct in vivo evidence for the dopaminergic molecular basis of functional recovery in the lesioned nigrostriatal system after neural transplantation.

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