Phase-sensitive surface plasmon resonance biosensors: methodology, instrumentation and applications

Authors

  • Y.H. Huang,

    1. Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin, NT, Hong Kong
    2. Center for Environmental Sensing and Modeling, Singapore-MIT Alliance for Research and Technology, Singapore
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  • H.P. Ho,

    1. Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin, NT, Hong Kong
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  • S.K. Kong,

    1. Programme of Biochemistry, School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong
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  • A.V. Kabashin

    Corresponding author
    1. Laboratoire Lasers, Plasmas et Procédés Photoniques (LP3, UMR 7341 CNRS), Faculté des Sciences de Luminy, Aix-Marseille University, 163 Avenue de Luminy, C. P. 917, 13288 Marseille Cedex 09, France
    • Laboratoire Lasers, Plasmas et Procédés Photoniques (LP3, UMR 7341 CNRS), Faculté des Sciences de Luminy, Aix-Marseille University, 163 Avenue de Luminy, C. P. 917, 13288 Marseille Cedex 09, France
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    • Phone: 33-4-9182-9383, Fax: 33-4-9182-9189


Abstract

Surface Plasmon Resonance (SPR) has become a central tool for label-free characterization of biomolecular interactions. Based on monitoring of amplitude characteristics, conventional SPR sensors have been extensively explored, commercialized and applied for studies of many important interactions (antigen-antibody, protein-ligand etc), but this technology still lacks of sensitivity for the detection of relatively small and low copy number compounds. Phase-sensitive SPR has recently emerged as an upgrade of this technology to resolve the sensitivity issue. Profiting from a sharp phase jump under SPR and ultra-sensitive tools of its control, this technology offers up to 100-time improvement of the detection limit, giving access to the detection of trace amounts of small molecular weight analytes (drugs etc). This paper intends to provide a tutorial on basic concepts of phase detection in SPR sensing, compare the performance of phase- and amplitude-sensitive sensors, review recent progress in the development and applications of phase-sensitive SPR sensors, and outline future prospects and trends of this technology.

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