Advertisement

Structural Analysis of 1-Aminocyclopropane-1-Carboxylate Deaminase: Observation of an Aminyl Intermediate and Identification of Tyr 294 as the Active-Site Nucleophile

Authors

  • Subramanian Karthikeyan Dr.,

    1. Division of Medicinal Chemistry, College of Pharmacy and Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA, Fax: (+1) 612-626-7541
    Search for more papers by this author
  • Zongbao Zhao Dr.,

    1. Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390-9038, USA, Fax: (+1) 214-648-9099
    2. Current address: Dalian Institute of Chemical Physics, Dalian 116023, P.R. China
    Search for more papers by this author
  • Chai-lin Kao Dr.,

    1. Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390-9038, USA, Fax: (+1) 214-648-9099
    Search for more papers by this author
  • Qingxian Zhou,

    1. Division of Medicinal Chemistry, College of Pharmacy and Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA, Fax: (+1) 612-626-7541
    Search for more papers by this author
  • Zhihua Tao,

    1. Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390-9038, USA, Fax: (+1) 214-648-9099
    Search for more papers by this author
  • Hong Zhang Prof. Dr.,

    1. Division of Medicinal Chemistry, College of Pharmacy and Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA, Fax: (+1) 612-626-7541
    Search for more papers by this author
  • Hung-wen Liu Prof. Dr.

    1. Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390-9038, USA, Fax: (+1) 214-648-9099
    Search for more papers by this author

  • The authors gratefully acknowledge financial support provided by the National Institutes of Health (Grant no. GM40541 to H.-w.L., and no. GM63689 to H.Z.). We also thank Dr. Howard Robinson at Brookhaven National Laboratory for helping to collect the diffraction data.

Abstract

original image

Die Angriffsstelle finden: Strukturstudien an 1-Aminocyclopropan-1-carboxylat(ACC)-Desaminase im Komplex mit dem fest bindenden Inhibitor 1-Aminocyclopropanphosphonat (ACP, siehe Bild) ergaben das Auftreten eines Aminyladdukts als Intermediat. Die Struktur des Enzyms im Kristall und Mutationsstudien sprechen dafür, dass ein nucleophiler Angriff durch Tyr 294 die ACC-Ringöffnung auslöst.

Ancillary