Single DNA Rotaxanes of a Transmembrane Pore Protein

Authors

  • Jorge Sánchez-Quesada Dr.,

    1. Departments of Chemistry and Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-2798
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  • Alan Saghatelian Dr.,

    1. Departments of Chemistry and Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-2798
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  • Stephen Cheley Dr.,

    1. Department of Medical Biochemistry and Genetics, Texas A&M Health Science Center, 440 Reynolds Medical Building, College Station, TX 77843-1114, USA
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  • Hagan Bayley Prof. Dr.,

    1. Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, OX1 3TA, UK
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  • M. Reza Ghadiri Prof. Dr.

    1. Departments of Chemistry and Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-2798
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  • This work was supported by a grant from the Office of Naval Research (Grant no. MURI-99, N000149910717).

Abstract

original image

Biochemisches Nadelöhr: DNA-Poly(ethylenglycol)(DNA-PEG)-Hybridstränge werden in einer gewünschten Orientierung in eine α-Hämolysin-Transmembranpore eingefädelt (siehe Schema). Die erhaltenen Einzelmolekül-Rotaxane sind stabil und können – abhängig vom angelegten Potential, von der Orientierung, der Synthesemethode und der Stoppergruppe – zwischen zwei Zuständen reversibel geschaltet werden.

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