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An Exceptionally Selective Lead(II)-Regulatory Protein from Ralstonia Metallidurans: Development of a Fluorescent Lead(II) Probe†

  1. Peng Chen Prof.1,
  2. Bill Greenberg2,
  3. Safiyh Taghavi Dr.2,
  4. Christine Romano1,
  5. Daniel van der Lelie Dr.2,
  6. Chuan He1

Article first published online: 31 MAR 2005

DOI: 10.1002/ange.200462443

Issue

Angewandte Chemie

Angewandte Chemie

Volume 117, Issue 18, pages 2775–2779, April 29, 2005

Additional Information

How to Cite

Chen, P., Greenberg, B., Taghavi, S., Romano, C., van der Lelie, D. and He, C. (2005), An Exceptionally Selective Lead(II)-Regulatory Protein from Ralstonia Metallidurans: Development of a Fluorescent Lead(II) Probe. Angewandte Chemie, 117: 2775–2779. doi: 10.1002/ange.200462443

Author Information

  1. 1

    Department of Chemistry, The University of Chicago, 5735 S. Ellis Avenue, Chicago, IL 60637, USA, Fax: (+1) 773-702-0805

  2. 2

    Biology Department, Building 463, Brookhaven National Laboratory, 50 Bell Avenue, Upton, NY 11973-5000, USA

  1. †

    This work was supported by the University of Chicago and a Searle Scholar award to C.H. P.C. is supported by the Burroughs Wellcome Fund Cross-Disciplinary Training Program (grant no.: 1001774C). The work by B.G., S.T., and D.v.d.L. was supported by Laboratory Directed Research and Development funds at the Brookhaven National Laboratory under contract with the US Department of Energy. We thank Dr. J. Piccirilli for the use of the DNA synthesizer, Dr. N.-S. Li, E. M. Duguid, and Y. Mishina for assistance with DNA synthesis, and the Biophysics Core Facility at the University of Chicago, and Dr. M. A. Yousef for help with fluorescence and isothermal titration calorimetry measurements.

Publication History

  1. Issue published online: 25 APR 2005
  2. Article first published online: 31 MAR 2005
  3. Manuscript Revised: 3 FEB 2005
  4. Manuscript Received: 27 OCT 2004

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Keywords:

  • Binden von Blei;
  • Fluoreszenzsonden;
  • PbrR;
  • Regulatorische Proteine;
  • Sensoren

Graphical Abstract

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Blei-Glanz: Ein Pb2+-bindendes Protein aus Ralstonia metallidurans stand Modell für eine hoch empfindliche Pb2+-Fluoreszenzsonde (PbR691, türkise Kreise im Schema). Dieses Fluoreszenzsystem bescheinigt dem Protein eine überraschend hohe Selektivität für Pb2+ gegenüber anderen Metallionen.

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