An Exceptionally Selective Lead(II)-Regulatory Protein from Ralstonia Metallidurans: Development of a Fluorescent Lead(II) Probe

Authors


  • This work was supported by the University of Chicago and a Searle Scholar award to C.H. P.C. is supported by the Burroughs Wellcome Fund Cross-Disciplinary Training Program (grant no.: 1001774C). The work by B.G., S.T., and D.v.d.L. was supported by Laboratory Directed Research and Development funds at the Brookhaven National Laboratory under contract with the US Department of Energy. We thank Dr. J. Piccirilli for the use of the DNA synthesizer, Dr. N.-S. Li, E. M. Duguid, and Y. Mishina for assistance with DNA synthesis, and the Biophysics Core Facility at the University of Chicago, and Dr. M. A. Yousef for help with fluorescence and isothermal titration calorimetry measurements.

Abstract

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Blei-Glanz: Ein Pb2+-bindendes Protein aus Ralstonia metallidurans stand Modell für eine hoch empfindliche Pb2+-Fluoreszenzsonde (PbR691, türkise Kreise im Schema). Dieses Fluoreszenzsystem bescheinigt dem Protein eine überraschend hohe Selektivität für Pb2+ gegenüber anderen Metallionen.

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