Total Chemical Synthesis and X-ray Crystal Structure of a Protein Diastereomer: [D-Gln 35]Ubiquitin

Authors

  • Duhee Bang,

    1. Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637, USA, Fax: (+1) 773-702-0439
    2. Department of Chemistry, The University of Chicago, Chicago, IL 60637, USA
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  • George I. Makhatadze Prof.,

    1. Department of Biochemistry and Molecular Biology, Pennsylvania State University, Hershey, PA 17033, USA
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  • Valentina Tereshko,

    1. Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637, USA, Fax: (+1) 773-702-0439
    2. Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA
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  • Anthony A. Kossiakoff Prof.,

    1. Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637, USA, Fax: (+1) 773-702-0439
    2. Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA
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  • Stephen B. Kent Prof.

    1. Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637, USA, Fax: (+1) 773-702-0439
    2. Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA
    3. Department of Chemistry, The University of Chicago, Chicago, IL 60637, USA
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  • D.B. was supported by funding from the NSF MRSEC program at the University of Chicago (grant DMR 0213745). We acknowledge funding from DOE GTL program grant no. DE-FG02–04ER63786 (to S.B.K) and NIH grant no. GM54537 (to G.I.M). Portions of this work were performed at the DND-CAT (sector 5-ID) and IMCA-CAT (sector 17-ID) of the Advanced Photon Source. Use of the Advanced Photon Source was supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences, under contract no. W-31–109-Eng-38. Use of the DND-CAT beamline is supported by the E.I. DuPont de Nemours, The Dow Chemical Company, the US National Science Foundation through grant no. DMR-9304725, and the State of Illinois through the Department of Commerce and the Board of Higher Education Grant IBHE HECA NWU 96. Use of the IMCA-CAT beamline at the Advanced Photon Source was supported by the companies of the Industrial Macromolecular Crystallography Association through a contract with the Illinois Institute of Technology.

Abstract

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Verblüffende Ähnlichkeit: Eine effiziente Synthese von nativem Ubiquitin und seinem Diastereomer [D-Gln35]ubiquitin gelang durch die Kombination einer chemischen Ligation als Eintopfverfahren mit Proteinentschwefelung. Hochauflösende röntgenkristallographische Studien am Proteindiastereomer (siehe Bild) enthüllten verblüffend ähnliche Molekülstrukturen.

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