2,4-Dinitrobenzenesulfonyl Fluoresceins as Fluorescent Alternatives to Ellman's Reagent in Thiol-Quantification Enzyme Assays

Authors

  • Hatsuo Maeda Prof.,

    1. Division of Analytical Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University, 1–6 Yamada-oka, Suita, Osaka 565-0871, Japan, Fax: (+81) 6-6879-8206
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  • Hiromi Matsuno,

    1. Division of Analytical Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University, 1–6 Yamada-oka, Suita, Osaka 565-0871, Japan, Fax: (+81) 6-6879-8206
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  • Mai Ushida,

    1. Division of Analytical Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University, 1–6 Yamada-oka, Suita, Osaka 565-0871, Japan, Fax: (+81) 6-6879-8206
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  • Kohei Katayama,

    1. Division of Analytical Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University, 1–6 Yamada-oka, Suita, Osaka 565-0871, Japan, Fax: (+81) 6-6879-8206
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  • Kanako Saeki,

    1. Division of Analytical Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University, 1–6 Yamada-oka, Suita, Osaka 565-0871, Japan, Fax: (+81) 6-6879-8206
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  • Norio Itoh

    1. Division of Analytical Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University, 1–6 Yamada-oka, Suita, Osaka 565-0871, Japan, Fax: (+81) 6-6879-8206
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  • The authors thank Eisai Co., Ltd. (Tokyo, Japan) for the generous gift of donepezil. This work was supported in part by a Grant-in-Aid for Scientific Research (B 15390012) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

Abstract

original image

Fluoreszenzsensor für Thiole: Das Entschützen von nichtfluoreszierendem 1 durch Thiole (R′SH) verläuft in wässriger Lösung (pH 7.4) rasch und liefert nahezu quantitativ stark fluoreszierendes 2. Assays in Gegenwart von 1 sind somit eine schnelle und einfache Methode, um die Inhibierungskonstanten von Inhibitoren wie Donepezil gegenüber Acetyl- und Butyrylcholinesterasen zu bestimmen.

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