Get access

Label-Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer-Based Sensor

Authors

  • Yi Xiao Dr.,

    1. Department of Physics, Materials Department, and Institute for Polymers and Organic Solids, University of California, Santa Barbara, CA 93106, USA
    Search for more papers by this author
  • Arica A. Lubin,

    1. Department of Chemistry and Biochemistry and Program in BioMolecular Science and Engineering, University of California, Santa Barbara, CA 93106, USA, Fax: (+1) 805-893-4120
    Search for more papers by this author
  • Alan J. Heeger Prof.,

    1. Department of Physics, Materials Department, and Institute for Polymers and Organic Solids, University of California, Santa Barbara, CA 93106, USA
    Search for more papers by this author
  • Kevin W. Plaxco Prof.

    1. Department of Chemistry and Biochemistry and Program in BioMolecular Science and Engineering, University of California, Santa Barbara, CA 93106, USA, Fax: (+1) 805-893-4120
    Search for more papers by this author

  • This work was supported in part by the National Institutes of Health (Grant no.: EB002046) and by the Institute for Collaborative Biotechnologies (Grant no.: DAAD19-03-D-0004) from the U.S. Army Research Office. We gratefully acknowledge Brian Baker and Kevin Cash for providing critical commentary and Professor Pierre Petroff for his support.

Abstract

original image

Blau, Gold und DNA: Ein mit Methylenblau (MB) markiertes, Thrombin-bindendes DNA-Aptamer auf einer Goldoberfläche erfährt bei der Substratbindung eine ausgeprägte Konformationsänderung (siehe schematische Darstellung; eT: Elektronentransfer). Dieser Faltungsvorgang hat eine große, einfach zu messende Änderung im Redoxstrom zur Folge und ermöglicht so den elektrochemischen Nachweis von Thrombin im Blutserum.

Ancillary