Design of a Mechanism-Based Probe for Neuraminidase To Capture Influenza Viruses


  • Influenza A virus (A/WSN/33) was a gift from Dr. Shin-Ru Shih, Chang Gung University, Taiwan) and polyclonal anti-FluA antibody was a gift from Dr. Hour-Young Chen (Center for Disease Control Taiwan). Japanese encephalitis virus (JEV, Taiwanese strain, RP-9) and the mouse monoclonal antibody specific for this virus were kindly provided by Dr. Yi-Ling Lin (IBMS Academia Sinica). Zanamivir was a gift from GlaxoWellcome Research and Development Ltd. (Stevenage, UK) and from Prof. Ching-Shih Chen (The Ohio State University, OH). We thank Prof. Yulin Lam for proofreading the manuscript. This work was supported by the National Science Council (Grant nos. NSC 92-2113M-001-023 to S.-H.W. and NSC 93-3112-B-002-001 to L.-C.L.).


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Grippe im Griff: Die mechanismusbasierte Sonde 1 bildet ein biotinyliertes Addukt mit Neuraminidase (NA) aus Arthrobacter ureafaciens und inhibiert überdies eine Reihe weiterer NA-Aktivitäten. ELISA-Experimente bestätigen, dass Influenza-Viren von dieser Sonde selektiv gebunden werden.