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Synthetic Substrates for an Endoplasmic Reticulum Protein-Folding Sensor, UDP-Glucose: Glycoprotein Glucosyltransferase

Authors

  • Kiichiro Totani Dr.,

    1. RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
    2. CREST, Japan Science and Technology Agency (JST), Kawaguchi, Saitama 332-1102, Japan, Fax: (+81) 48-482-4680
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  • Yoshito Ihara Dr.,

    1. Department of Biochemistry & Molecular Biology of Disease, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
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  • Ichiro Matsuo Dr.,

    1. RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
    2. CREST, Japan Science and Technology Agency (JST), Kawaguchi, Saitama 332-1102, Japan, Fax: (+81) 48-482-4680
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  • Hiroyuki Koshino Dr.,

    1. RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
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  • Yukishige Ito Dr.

    1. RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
    2. CREST, Japan Science and Technology Agency (JST), Kawaguchi, Saitama 332-1102, Japan, Fax: (+81) 48-482-4680
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  • This work was supported by the special postdoctoral researchers program at RIKEN. We thank A. Takahashi for technical assistance. Financial support from the Presidential Fund of RIKEN and the Ministry of Education, Culture, Sports, Science, and Technology (Grant-in-Aid for Young Scientists (B) No. 17750170 and Grant-in-Aid for Creative Scientific Research No. 17GS0420) is acknowledged. UDP=uridine 5′-diphosphate.

Abstract

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In der Qualitätskontrolle von Glycoproteinen fungiert UDP-Glucose:Glycoprotein-Glucosyltransferase (UGGT) als Faltungssensor. Es glucosyliert Man9GlcNAc2 falsch gefalteter Glycoproteine zu Glc1Man9GlcNAc2, das ein Ligand von Calnexin und Calreticulin ist. Das synthetische Substrat Man9GlcNAc2-MTX kann zur quantitativen Analyse von UGGT verwendet werden. UDP=Uridin-5′-diphosphat, Glc=D-Glucose, Man= D-Mannose, GlcNAc=N-Acetyl- D-glucosamin, MTX=Methotrexat.

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