Get access

Site-Specific Protein Modification through CuI-Catalyzed 1,2,3-Triazole Formation and Its Implementation in Protein Microarray Fabrication

Authors

  • Po-Chiao Lin,

    1. Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan, Fax: (+886) 3-571-1082
    2. Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan
    3. Institute of Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan
    Search for more papers by this author
  • Shau-Hua Ueng Dr.,

    1. Institute of Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan
    Search for more papers by this author
  • Mei-Chun Tseng,

    1. Institute of Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan
    Search for more papers by this author
  • Jia-Ling Ko,

    1. Institute of Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan
    2. Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan
    Search for more papers by this author
  • Kuo-Ting Huang,

    1. Institute of Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan
    Search for more papers by this author
  • Sheng-Chieh Yu,

    1. Institute of Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan
    Search for more papers by this author
  • Avijit Kumar Adak Dr.,

    1. Institute of Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan
    Search for more papers by this author
  • Yu-Ju Chen Prof.,

    1. Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan, Fax: (+886) 3-571-1082
    2. Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan
    3. Institute of Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan
    Search for more papers by this author
  • Chun-Cheng Lin Prof.

    1. Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan, Fax: (+886) 3-571-1082
    2. Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan
    3. Institute of Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan
    Search for more papers by this author

  • We acknowledge financial support from the National Tsing Hua University (Academia Sinica Research Project on Nano Science and Technology) and the National Science Council, Taiwan.

Abstract

original image

Gezielt ligiert: Ein Proteinexpressionssystem wurde mit der CuI-katalysierten Bildung von 1,2,3-Triazolen kombiniert, um ein Protein an seinem C-Terminus zu modifizieren. Das immobilisierte Stammprotein lässt sich mit kleinen Molekülen ortsspezifisch ligieren (siehe Bild), wobei die Ligation mit einem Diazido-Linker zu homodimeren Proteinkonjugaten führt. Durch spezifische kovalente Anbindung an funktionalisierte Oberflächen können außerdem Mikroarrays erzeugt werden.

Get access to the full text of this article

Ancillary