Inhibition of siRNA Binding to a p19 Viral Suppressor of RNA Silencing by Cysteine Alkylation

Authors

  • Selena M. Sagan,

    1. The Steacie Institute for Molecular Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, K1H 0R6, Canada, Fax: (+1) 613-952-0068
    2. Department of Biochemistry, Microbiology & Immunology/Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, K1H 8M5, Canada
    Search for more papers by this author
    • These authors contributed equally to this research.

  • Roger Koukiekolo Dr.,

    1. The Steacie Institute for Molecular Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, K1H 0R6, Canada, Fax: (+1) 613-952-0068
    Search for more papers by this author
    • These authors contributed equally to this research.

  • Elisabeth Rodgers,

    1. The Steacie Institute for Molecular Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, K1H 0R6, Canada, Fax: (+1) 613-952-0068
    Search for more papers by this author
  • Natalie K. Goto Prof.,

    1. Department of Chemistry, University of Ottawa, Ottawa, K1N 6N5, Canada
    2. Department of Biochemistry, Microbiology & Immunology/Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, K1H 8M5, Canada
    Search for more papers by this author
  • John Paul Pezacki Prof.

    1. The Steacie Institute for Molecular Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, K1H 0R6, Canada, Fax: (+1) 613-952-0068
    2. Department of Chemistry, University of Ottawa, Ottawa, K1N 6N5, Canada
    3. Department of Biochemistry, Microbiology & Immunology/Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, K1H 8M5, Canada
    Search for more papers by this author

  • We thank Y. Rouleau and S. Bélanger for assistance with RNA preparation, P. Bouchard (NRC-BRI) for providing compounds from the Tripos Optiverse Panlab library, and the Genomics and Health Initiative of the NRC for partial funding of this research. S.M.S. thanks the Natural Sciences and Engineering Research Council for funding in the form of a Graduate Scholarship.

Abstract

original image

Eukaryoten zeigen komplexe Reaktionen auf doppelsträngige RNA. Die Menge an siRNA (small interfering RNA) lässt sich schnell mithilfe des p19-Proteins von CIRV (carnation Italian ringspot virus) in 96er Lochplatten bestimmen. CIRV 19 bindet mit nanomolarer Affinität an doppelsträngige siRNAs und unterscheidet sie nach ihrer Länge. Zwei Verbindungen hemmen diese Bindung durch Alkylierung von Cysteinresten in den aktiven Zentren (siehe Bild).

Ancillary