Functional Architecture of HCV IRES Domain II Stabilized by Divalent Metal Ions in the Crystal and in Solution

Authors

  • Sergey M. Dibrov Dr.,

    1. Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA, Fax: (+1) 858-534-0202
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  • Hillary Johnston-Cox,

    1. Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA, Fax: (+1) 858-534-0202
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  • Yi-Hsin Weng,

    1. Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA, Fax: (+1) 858-534-0202
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  • Thomas Hermann Dr.

    1. Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA, Fax: (+1) 858-534-0202
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  • We thank N. Nguyen for assistance with crystallographic data collection, Dr. C. Kim for help with data processing, and J. Parsons for help with UV melting experiments. This work was supported by faculty startup funds from the University of California, San Diego. HCV=hepatitis C virus, IRES=internal ribosome entry site.

Abstract

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Um die Kurve: Eine rechtwinklige Krümmung in der RNA des Hepatitis-C-Virus wird durch einen Kern von zweiwertigen Metallionen stabilisiert. Mithilfe einer Kristallstrukturanalyse und einer Fluoreszenzmarkierung wurden die Struktur und die metallionenabhängige Stabilisierung der RNA-Domäne untersucht, die eine zentrale Rolle bei der viralen Proteinsynthese spielt.

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