Zuschrift

Silica Immobilization of an Enzyme through Genetic Engineering of the Diatom Thalassiosira pseudonana†

  1. Nicole Poulsen Dr.,
  2. Cécile Berne Dr.2,4,
  3. Jim Spain Prof. Dr.3,
  4. Nils Kröger Prof. Dr.1

Article first published online: 2 FEB 2007

DOI: 10.1002/ange.200603928

Issue

Angewandte Chemie

Angewandte Chemie

Volume 119, Issue 11, pages 1875–1878, March 5, 2007

Additional Information

How to Cite

Poulsen, N., Berne, C., Spain, J. and Kröger, N. (2007), Silica Immobilization of an Enzyme through Genetic Engineering of the Diatom Thalassiosira pseudonana. Angew. Chem., 119: 1875–1878. doi: 10.1002/ange.200603928

Author Information

  1. 1

    School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332-0400, USA, Fax: (+1) 404-894-7452

  2. 2

    Airforce Research Laboratory, 139 Barnes Drive, Tyndall AFB, FL 32403, USA

  3. 3

    School of Civil and Environmental Engineering, Georgia Institute of Technology, 331 Ferst Drive Northwest, Atlanta, GA 30332-0512, USA

  4. 4

    Present address: Department of Biology, Indiana University, 1001 East Third Street, Bloomington, IN 47405-3700, USA

  1. †

    We thank Christoph Fahrni for help with analysis of the kinetics of GFP photobleaching, Danielle Armstrong for cloning and expression of GFPHis6, and Andrew Conley for help with DNA cloning. This work was supported by grants from the Office of Naval Research (to N.K.), the Defense Advanced Research Projects Agency (to N.K.), and the Air Force Office of Scientific Research (to J.S.).

Publication History

  1. Issue published online: 26 FEB 2007
  2. Article first published online: 2 FEB 2007
  3. Manuscript Received: 25 SEP 2006

Funded by

  • Office of Naval Research
  • Defense Advanced Research Projects Agency
  • Air Force Office of Scientific Research

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Keywords:

  • Diatomeen;
  • Enzymkatalyse;
  • Gentechnik;
  • Immobilisierung;
  • Siliciumoxide
Thumbnail image of graphical abstract

Biosilica-Strukturen, die Fremdproteine mit gewünschten Funktionen enthalten, können molekulargenetisch hergestellt werden. Ein Enzym, das in Diatomeen-Siliciumoxid immobilisiert ist, war stabiler als gereinigte Hydroxylaminobenzol-Mutase in Lösung. Derartig immobilisierte aktive Biomoleküle könnten zur Herstellung von Sensormaterialien und wiederverwendbaren Katalysatoren genutzt werden.

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