The Mechanism of RNA Strand Scission: An Experimental Measure of the Brønsted Coefficient, βnuc

Authors

  • Jing-Dong Ye Dr.,

    1. Howard Hughes Medical Institute, Department of Chemistry, Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA, Fax: (+1) 773-702-0271
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  • Nan-Sheng Li,

    1. Howard Hughes Medical Institute, Department of Chemistry, Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA, Fax: (+1) 773-702-0271
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  • Qing Dai,

    1. Howard Hughes Medical Institute, Department of Chemistry, Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA, Fax: (+1) 773-702-0271
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  • Joseph A. Piccirilli Prof. Dr.

    1. Howard Hughes Medical Institute, Department of Chemistry, Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA, Fax: (+1) 773-702-0271
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  • This research was supported by the Howard Hughes Medical Institute.

Abstract

original image

RNA-Spaltung näher betrachtet: Fluorierte Nucleotidanaloga (X) wurden in RNA eingeführt, um den pKS-Wert der 2′-OH-Gruppe systematisch zu modifizieren. Dies bietet Möglichkeiten, die Bindung von RNA an das Nucleophil während der RNA-Spaltung mit physikalisch-organischen Methoden zu untersuchen. Der Brønsted-Koeffizient βnuc liefert Informationen zur Struktur des Übergangszustands dieser lange bekannten Reaktion.

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