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Auxiliary-Mediated Site-Specific Peptide Ubiquitylation

Authors

  • Champak Chatterjee Dr.,

    1. The Laboratory of Synthetic Protein Chemistry, The Rockefeller University, Box 223, 1230 York Avenue, New York, NY 10021, USA, Fax: (+1) 212-327-7358
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  • Robert K. McGinty,

    1. The Laboratory of Synthetic Protein Chemistry, The Rockefeller University, Box 223, 1230 York Avenue, New York, NY 10021, USA, Fax: (+1) 212-327-7358
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  • Jean-Philippe Pellois Dr.,

    1. The Laboratory of Synthetic Protein Chemistry, The Rockefeller University, Box 223, 1230 York Avenue, New York, NY 10021, USA, Fax: (+1) 212-327-7358
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  • Tom W. Muir Prof.

    1. The Laboratory of Synthetic Protein Chemistry, The Rockefeller University, Box 223, 1230 York Avenue, New York, NY 10021, USA, Fax: (+1) 212-327-7358
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  • We thank Leonard S. Smith III for providing the ubiquitin plasmid and Baldissera Giovani for assistance with cloning. We would also like to thank Prof. C. David Allis and Jason Tanny for many stimulating discussions. This work was supported by the U.S. National Institutes of Health. R.K.M. was supported by an MSTP grant (GM 07739).

Abstract

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1) Ubiquitin dran! 2) Licht an! Ein photolytisch abspaltbares Auxiliar (Aux) half beim ortsspezifischen Anbringen von Ubiquitin (Ub) an einen von drei Lysinresten in einem C-terminalen Peptidfragment von Histon H2B (cH2B). Das Ligationsprodukt war als Substrat für die Hydrolase für C-terminales Ubiquitin (UCH-L3) geeignet.

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