A Pocket-Sized Convective PCR Thermocycler

Authors

  • Nitin Agrawal Dr.,

    1. Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA, Fax: (+1) 979-458-1002
    2. Present address: Center for Engineering in Medicine/Department of Surgery, Massachusetts General Hospital, Shriners Burns Hospital and Harvard Medical School, Boston, MA 02114, USA
    Search for more papers by this author
  • Yassin A. Hassan Prof.,

    1. Department of Nuclear Engineering, Texas A&M University, College Station, TX 77843, USA
    Search for more papers by this author
  • Victor M. Ugaz Prof.

    1. Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA, Fax: (+1) 979-458-1002
    Search for more papers by this author

  • This work was supported by the National Institutes of Health under grants K22-HG02297 and R01-HG003364. We thank Prof. Mihir Sen for helpful discussions regarding modeling the convective flow. We are also grateful to Ling Zhen for assistance with computational-flow simulations.

Abstract

original image

Taschen-PCR: Ein innovativer Thermocycler nutzt die natürliche Konvektion, um langkettige und Multiplex-DNA schnell durch Polymerasekettenreaktion (PCR) zu vervielfältigen. Der einfache Aufbau benötigt ein Minimum an elektrischer Leistung und kann daher in einem batteriebetriebenen Tascheninstrument mit Produktionskosten um 10 US$ umgesetzt werden.

Ancillary