Protein Kinase Assay on Peptide-Conjugated Gold Nanoparticles by Using Secondary-Ion Mass Spectrometric Imaging

Authors

  • Young-Pil Kim,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Korea, Fax: (+82) 42-869-2610
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  • Eunkeu Oh Dr.,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Korea, Fax: (+82) 42-869-2610
    2. Current address: Samsung SDI Co. 428-5 Gongse-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 446-577, Korea
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  • Young-Hee Oh,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Korea, Fax: (+82) 42-869-2610
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  • Dae Won Moon Dr.,

    1. Nanobio Fusion Research Center, Korea Research Institute of Standards and Science, Daejeon 305-600, Korea, Fax: (+82) 42-868-5032
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  • Tae Geol Lee Dr.,

    1. Nanobio Fusion Research Center, Korea Research Institute of Standards and Science, Daejeon 305-600, Korea, Fax: (+82) 42-868-5032
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  • Hak-Sung Kim Prof.

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Korea, Fax: (+82) 42-869-2610
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  • This work was supported by the Bio-Signal Analysis Technology Innovation Program (M106450100002-06N4501-00210), the Nano/Bio Science & Technology Program (M10503000218-05M0300-21810), and the Nano Science & Technology Program (M10536090002-07N3609-00210) of MOST, the Korea Health 21C R&D Project (0405-MN01-0604-0007) of MHW, and the Next-Generation New-Technology Development Program for MOCIE.

Abstract

original image

Peptide massenweise: Ein markierungsfreier Proteinkinase-Assay mit peptidkonjugierten Goldnanopartikeln (AuNPs) unter Bildgebung mit Sekundärionen-Massenspektrometrie wurde entwickelt. Bei der Detektion der Massenänderung von Peptidsubstraten in einer Kinasereaktion ermöglichen die AuNP-verstärkten Peptidsignale die Untersuchung der Proteinkinase und ihrer Inhibierung durch chemische Bildgebung der Substrate auf einer Oberfläche (siehe Bild).

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