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A Biosynthetic Route to Dehydroalanine-Containing Proteins

Authors

  • Jiangyun Wang Dr.,

    1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440
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  • Stefan M. Schiller Dr.,

    1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440
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  • Peter G. Schultz Prof.

    1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440
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  • We thank Dr. M. Sever and Dr. J. Guo for helpful discussions. We thank Bill Webb for help in protein mass spectroscopy. We are grateful to the DOE (ER46051) and the Skaggs Institute for Chemical Biology for support.

Abstract

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Die nichtnatürliche Aminosäure Phenylselenocystein wurde in E. coli als Antwort auf das Amber-Stoppcodon TAG gentechnisch in Proteine eingefügt. Durch oxidative Eliminierung und anschließende Michael-Addition wurden (S)-Hexadecyl- und (S)-Mannosylcystein in grün fluoreszierendes Protein eingebaut. Mit dieser Methode können die Seitenketten und das Rückgrat von Proteinen modifiziert werden.

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