Specific DNA Recognition by a Synthetic, Monomeric Cys2His2 Zinc-Finger Peptide Conjugated to a Minor-Groove Binder

Authors

  • Olalla Vázquez,

    1. Dept. de Química Orgánica y Unidad Asociada al CSIC, Universidade de Santiago de Compostela, Facultade de Química, 15782 Santiago de Compostela, Spain, Fax: (+34) 981-595-012
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  • M. Eugenio Vázquez Dr.,

    1. Dept. de Química Orgánica y Unidad Asociada al CSIC, Universidade de Santiago de Compostela, Facultade de Química, 15782 Santiago de Compostela, Spain, Fax: (+34) 981-595-012
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  • Juan B. Blanco Dr.,

    1. Dept. de Química Orgánica y Unidad Asociada al CSIC, Universidade de Santiago de Compostela, Facultade de Química, 15782 Santiago de Compostela, Spain, Fax: (+34) 981-595-012
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  • Luis Castedo Prof. Dr.,

    1. Dept. de Química Orgánica y Unidad Asociada al CSIC, Universidade de Santiago de Compostela, Facultade de Química, 15782 Santiago de Compostela, Spain, Fax: (+34) 981-595-012
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  • José L. Mascareñas Prof. Dr.

    1. Dept. de Química Orgánica y Unidad Asociada al CSIC, Universidade de Santiago de Compostela, Facultade de Química, 15782 Santiago de Compostela, Spain, Fax: (+34) 981-595-012
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  • This work was supported by the Spanish MEC (SAF2004-01044) and the Xunta de Galicia (PGIDIT04TF209006PR, PGIDIT06PXIB209018PR, and GRC2006/132). O.V. and J.B.B. thank the Spanish M.E.C. for their PhD fellowships. M.E.V. thanks the Human Frontier Science Program Organization for their support with a Career Development Award (CDA0032/2005-C) and the Spanish MEC for a “Ramon y Cajal” contract. We also thank Prof. J. Benavente for access to radioactivity facilities.

Abstract

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Um den Finger gewickelt: Maßgeschneiderte Cys2His2-Zinkfingerdomänen können spezifisch an DNA binden, wenn sie durch Wechselwirkungen in der kleinen Furche unterstützt werden. Entsprechend binden passend entworfene Hybride, die die Zinkfingereinheit des GAGA-Faktors und ein Tripyrrol vom Distamintyp enthalten, mit hoher Affinität an DNA-Positionen, in denen eine GAG-Triade nahe am 3′-Ende eines A-reichen Fragments vorliegt.

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