Get access

Photochemical Surface Patterning by the Thiol-Ene Reaction

Authors

  • Pascal Jonkheijm Dr.,

    1. Department of Chemical Biology and Department of Structural Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
    2. Faculty of Chemistry, Chemical Biology, Technical University of Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
    Search for more papers by this author
  • Dirk Weinrich Dipl.-Chem.,

    1. Department of Chemical Biology and Department of Structural Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
    2. Faculty of Chemistry, Chemical Biology, Technical University of Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
    Search for more papers by this author
  • Maja Köhn Dr.,

    1. Department of Chemical Biology and Department of Structural Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
    2. Faculty of Chemistry, Chemical Biology, Technical University of Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
    Search for more papers by this author
  • Hans Engelkamp Dr.,

    1. Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
    Search for more papers by this author
  • Peter C. M. Christianen Dr.,

    1. Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
    Search for more papers by this author
  • Jürgen Kuhlmann Dr.,

    1. Department of Chemical Biology and Department of Structural Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
    2. Faculty of Chemistry, Chemical Biology, Technical University of Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
    Search for more papers by this author
  • Jan C. Maan Prof. Dr.,

    1. Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
    Search for more papers by this author
  • Dirk Nüsse Dr.,

    1. Faculty of Chemistry, Biological-Chemical Microstructuring, Technical University of Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany, Fax: (+49) 231-755-7082
    Search for more papers by this author
  • Hendrik Schroeder Dr.,

    1. Faculty of Chemistry, Biological-Chemical Microstructuring, Technical University of Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany, Fax: (+49) 231-755-7082
    Search for more papers by this author
  • Ron Wacker Dr.,

    1. Faculty of Chemistry, Biological-Chemical Microstructuring, Technical University of Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany, Fax: (+49) 231-755-7082
    Search for more papers by this author
  • Rolf Breinbauer Prof. Dr.,

    1. Department of Chemical Biology and Department of Structural Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
    2. Faculty of Chemistry, Chemical Biology, Technical University of Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
    Search for more papers by this author
  • Christof M. Niemeyer Prof. Dr.,

    1. Faculty of Chemistry, Biological-Chemical Microstructuring, Technical University of Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany, Fax: (+49) 231-755-7082
    Search for more papers by this author
  • Herbert Waldmann Prof. Dr.

    1. Department of Chemical Biology and Department of Structural Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
    2. Faculty of Chemistry, Chemical Biology, Technical University of Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany, Fax: (+49) 231-133-2499
    Search for more papers by this author

  • We thank M. Brasch, C. Nowak, and E. Voges for assistance and discussions, the Max-Planck-Gesellschaft, the research program “Molecular Basics of Biosciences” of the Technical University of Dortmund, the Fonds der Chemischen Industrie, and the Zentrum für Angewandte Chemische Genomik for financial support. P.J. thanks the Alexander von Humboldt Foundation for a postdoctoral fellowship.

Abstract

original image

Das photochemische Kuppeln olefinbedeckter (Bio)moleküle an oberflächengebundene Thiole kann dazu dienen, die Immobilisierung von Proteinen im Zentimeter- bis Submikrometerbereich zu steuern (das Fluoreszenzmikroskopiebild zeigt ein Nanomuster nach der Behandlung mit markiertem Streptavidin). Zwei auf dem Muster immobilisierte Enzyme behielten ihre Aktivität und zeigten ähnliche Protein-Protein-Wechselwirkungen wie in Lösung.

Ancillary