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Molekulartechnische DNA-Modifizierung: Molecular Beacons

Authors

  • Kemin Wang Prof.,

    1. Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, UF Genetics Institute and Shands Cancer Center, University of Florida, Gainesville, FL 32611-7200 (USA), Fax: (+1) 352-846-2410
    2. Biomedical Engineering Center, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (P.R. China)
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  • Zhiwen Tang Dr.,

    1. Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, UF Genetics Institute and Shands Cancer Center, University of Florida, Gainesville, FL 32611-7200 (USA), Fax: (+1) 352-846-2410
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  • Chaoyong James Yang Dr.,

    1. Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
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  • Youngmi Kim Dr.,

    1. Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, UF Genetics Institute and Shands Cancer Center, University of Florida, Gainesville, FL 32611-7200 (USA), Fax: (+1) 352-846-2410
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  • Xiaohong Fang Prof.,

    1. Institute of Chemistry, Chinese Academy of Sciences, 2 Zhongguancun Beiyijie, Beijing 100190 (P.R. China)
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  • Wei Li,

    1. Biomedical Engineering Center, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (P.R. China)
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  • Yanrong Wu,

    1. Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, UF Genetics Institute and Shands Cancer Center, University of Florida, Gainesville, FL 32611-7200 (USA), Fax: (+1) 352-846-2410
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  • Colin D. Medley Dr.,

    1. Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, UF Genetics Institute and Shands Cancer Center, University of Florida, Gainesville, FL 32611-7200 (USA), Fax: (+1) 352-846-2410
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  • Zehui Cao Dr.,

    1. Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, UF Genetics Institute and Shands Cancer Center, University of Florida, Gainesville, FL 32611-7200 (USA), Fax: (+1) 352-846-2410
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  • Jun Li Dr.,

    1. Biomedical Engineering Center, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (P.R. China)
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  • Patrick Colon,

    1. Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, UF Genetics Institute and Shands Cancer Center, University of Florida, Gainesville, FL 32611-7200 (USA), Fax: (+1) 352-846-2410
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  • Hui Lin,

    1. Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, UF Genetics Institute and Shands Cancer Center, University of Florida, Gainesville, FL 32611-7200 (USA), Fax: (+1) 352-846-2410
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  • Weihong Tan Prof.

    1. Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, UF Genetics Institute and Shands Cancer Center, University of Florida, Gainesville, FL 32611-7200 (USA), Fax: (+1) 352-846-2410
    2. Biomedical Engineering Center, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (P.R. China)
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Abstract

Molecular Beacons (MBs) sind spezifisch entworfene DNA-Haarnadelstrukturen, die als Fluoreszenzsonden für verschiedenste Zwecke eingesetzt werden. Die Anwendungen reichen von genetischem Screening über die Herstellung von Biochips und den Nachweis von Einzelnucleotidpolymorphien bis hin zum mRNA-Monitoring in lebenden Zellen und der Untersuchung von Protein-Protein-Wechselwirkungen. Ermöglicht wird dieses breite Anwendungsspektrum durch die besondere Art, mit der MBs mit DNA-, RNA- und Proteinmolekülen wechselwirken. Durch die Haarnadelstruktur der MBs wird eine enge Nachbarschaft zwischen einem Fluoreszenzdonor und einem -akzeptor hergestellt, wodurch es zu einem resonanten Energietransfer kommt. Die Hybridisierung der Schleifenregion mit der Zielsequenz bewirkt eine Konformationsänderung der Sonden, die den Donor und Akzeptor auseinanderbewegt und die Fluoreszenz wieder herstellt. Die aktuelle Forschung zielt auf die Verbesserung der Sonden, die intrazelluläre Quantifizierung von Genen und die Anwendung in Studien von Protein-Protein-Wechselwirkungen.

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